1utb: Difference between revisions
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'''DNTR FROM BURKHOLDERIA SP. STRAIN DNT''' | '''DNTR FROM BURKHOLDERIA SP. STRAIN DNT''' | ||
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[[Category: Mcsweeney, S.]] | [[Category: Mcsweeney, S.]] | ||
[[Category: Smirnova, I A.]] | [[Category: Smirnova, I A.]] | ||
[[Category: | [[Category: Lysr]] | ||
[[Category: | [[Category: Transcriptional regulator]] | ||
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Revision as of 08:39, 3 May 2008
DNTR FROM BURKHOLDERIA SP. STRAIN DNT
Overview
The transcriptional regulator DntR, a member of the LysR family, is a central element in a prototype bacterial cell-based biosensor for the detection of hazardous contamination of soil and groundwater by dinitrotoluenes. To optimise the sensitivity of the biosensor for such compounds we have chosen a rational design of the inducer-binding cavity based on knowledge of the three-dimensional structure of DntR. We report two crystal structures of DntR with acetate (resolution 2.6 angstroms) and thiocyanate (resolution 2.3 angstroms), respectively, occupying the inducer-binding cavity. These structures allow for the construction of models of DntR in complex with salicylate (Kd approximately or = 4 microM) and 2,4-dinitrotoluene that provide a basis for the design of mutant DntR with enhanced specificity for dinitrotoluenes. In both crystal structures DntR crystallises as a homodimer with a "head-to-tail" arrangement of monomers in the asymmetric unit. Analysis of the crystal structure has allowed the building of a full-length model of DntR in its biologically active homotetrameric form consisting of two "head-to-head" dimers. The implications of this model for the mechanism of transcription regulation by LysR proteins are discussed.
About this Structure
1UTB is a Protein complex structure of sequences from Burkholderia sp.. Full crystallographic information is available from OCA.
Reference
Development of a bacterial biosensor for nitrotoluenes: the crystal structure of the transcriptional regulator DntR., Smirnova IA, Dian C, Leonard GA, McSweeney S, Birse D, Brzezinski P, J Mol Biol. 2004 Jul 9;340(3):405-18. PMID:15210343 Page seeded by OCA on Sat May 3 11:39:37 2008
