9iy8: Difference between revisions

Latest revision as of 14:03, 1 January 2025

Cryo-EM structure of apo-GPR55-G13 complex

Structural highlights

9iy8 is a 5 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.01Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GPR55_HUMAN G-protein coupled receptor that binds to several ligands including 2-arachidonoyl lysophosphatidylinositol or lysophosphatidylglucoside with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways (PubMed:36142844, PubMed:36523570, PubMed:37544935). Induces the Ca(2+) release from intracellular stores via ERK, the heterotrimeric G protein GNA13 and RHOA leading to morphological changes including cell rounding and stress fiber formation (PubMed:36142844). In macrophages, acts downstream of lysophosphatidylglucoside to inhibit the translocation of the phospholipid-transporting ABCA1 to plasma membrane and subsequent cholesterol efflux leading to lipid accumulation and foam cell formation (PubMed:37544935).^[1] ^[2] ^[3] ^[4]

References

↑ Whyte LS, Ryberg E, Sims NA, Ridge SA, Mackie K, Greasley PJ, Ross RA, Rogers MJ. The putative cannabinoid receptor GPR55 affects osteoclast function in vitro and bone mass in vivo. Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16511-6. PMID:19805329 doi:10.1073/pnas.0902743106
↑ Nakajima K, Oka S, Tanikawa T, Nemoto-Sasaki Y, Matsumoto N, Ishiguro H, Arata Y, Sugiura T, Yamashita A. Lysophosphatidylinositol Induced Morphological Changes and Stress Fiber Formation through the GPR55-RhoA-ROCK Pathway. Int J Mol Sci. 2022 Sep 18;23(18):10932. PMID:36142844 doi:10.3390/ijms231810932
↑ Guillamat-Prats R, Hering D, Derle A, Rami M, Härdtner C, Santovito D, Rinne P, Bindila L, Hristov M, Pagano S, Vuilleumier N, Schmid S, Janjic A, Enard W, Weber C, Maegdefessel L, Faussner A, Hilgendorf I, Steffens S. GPR55 in B cells limits atherosclerosis development and regulates plasma cell maturation. Nat Cardiovasc Res. 2022 Nov;1:1056-1071. PMID:36523570 doi:10.1038/s44161-022-00155-0
↑ Shimai R, Hanafusa K, Nakayama H, Oshima E, Kato M, Kano K, Matsuo I, Miyazaki T, Tokano T, Hirabayashi Y, Iwabuchi K, Minamino T. Lysophosphatidylglucoside/GPR55 signaling promotes foam cell formation in human M2c macrophages. Sci Rep. 2023 Aug 6;13(1):12740. PMID:37544935 doi:10.1038/s41598-023-39904-x

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Whyte LS, Ryberg E, Sims NA, Ridge SA, Mackie K, Greasley PJ, Ross RA, Rogers MJ. The putative cannabinoid receptor GPR55 affects osteoclast function in vitro and bone mass in vivo. Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16511-6. PMID:19805329 doi:10.1073/pnas.0902743106

[2] Nakajima K, Oka S, Tanikawa T, Nemoto-Sasaki Y, Matsumoto N, Ishiguro H, Arata Y, Sugiura T, Yamashita A. Lysophosphatidylinositol Induced Morphological Changes and Stress Fiber Formation through the GPR55-RhoA-ROCK Pathway. Int J Mol Sci. 2022 Sep 18;23(18):10932. PMID:36142844 doi:10.3390/ijms231810932

[3] Guillamat-Prats R, Hering D, Derle A, Rami M, Härdtner C, Santovito D, Rinne P, Bindila L, Hristov M, Pagano S, Vuilleumier N, Schmid S, Janjic A, Enard W, Weber C, Maegdefessel L, Faussner A, Hilgendorf I, Steffens S. GPR55 in B cells limits atherosclerosis development and regulates plasma cell maturation. Nat Cardiovasc Res. 2022 Nov;1:1056-1071. PMID:36523570 doi:10.1038/s44161-022-00155-0

[4] Shimai R, Hanafusa K, Nakayama H, Oshima E, Kato M, Kano K, Matsuo I, Miyazaki T, Tokano T, Hirabayashi Y, Iwabuchi K, Minamino T. Lysophosphatidylglucoside/GPR55 signaling promotes foam cell formation in human M2c macrophages. Sci Rep. 2023 Aug 6;13(1):12740. PMID:37544935 doi:10.1038/s41598-023-39904-x

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9iy8 is ON HOLD  until Paper Publication
+==Cryo-EM structure of apo-GPR55-G13 complex==
+<StructureSection load='9iy8' size='340' side='right'caption='[[9iy8]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9iy8]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9IY8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9IY8 FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.01&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9iy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9iy8 OCA], [https://pdbe.org/9iy8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9iy8 RCSB], [https://www.ebi.ac.uk/pdbsum/9iy8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9iy8 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GPR55_HUMAN GPR55_HUMAN] G-protein coupled receptor that binds to several ligands including 2-arachidonoyl lysophosphatidylinositol or lysophosphatidylglucoside with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways (PubMed:36142844, PubMed:36523570, PubMed:37544935). Induces the Ca(2+) release from intracellular stores via ERK, the heterotrimeric G protein GNA13 and RHOA leading to morphological changes including cell rounding and stress fiber formation (PubMed:36142844). In macrophages, acts downstream of lysophosphatidylglucoside to inhibit the translocation of the phospholipid-transporting ABCA1 to plasma membrane and subsequent cholesterol efflux leading to lipid accumulation and foam cell formation (PubMed:37544935).<ref>PMID:19805329</ref> <ref>PMID:36142844</ref> <ref>PMID:36523570</ref> <ref>PMID:37544935</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Chang H]]
+[[Category: Cherezov V]]
+[[Category: Hua T]]
+[[Category: Li XT]]
+[[Category: Liu ZJ]]
+[[Category: Shen L]]

9iy8: Difference between revisions

Latest revision as of 14:03, 1 January 2025

Cryo-EM structure of apo-GPR55-G13 complex

Structural highlights

Function

References

Contents

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