8hcj: Difference between revisions

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'''Unreleased structure'''


The entry 8hcj is ON HOLD  until Paper Publication
==Structure of GH43 family enzyme, Xylan 1, 4 Beta- xylosidase from pseudopedobacter saltans==
<StructureSection load='8hcj' size='340' side='right'caption='[[8hcj]], [[Resolution|resolution]] 2.57&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8hcj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudopedobacter_saltans_DSM_12145 Pseudopedobacter saltans DSM 12145]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HCJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.566&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hcj OCA], [https://pdbe.org/8hcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hcj RCSB], [https://www.ebi.ac.uk/pdbsum/8hcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hcj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F0S5E9_PSESL F0S5E9_PSESL]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Xylose, a key constituent of the heterogeneous hemicellulose polymer, occurs in lignocellulosic biomass and forms xylan polymers through beta-1,4 glycosidic linkages. The beta-1,4-xylosidase enzyme was isolated from Pseudopedobacter saltans (PsGH43) to find an effective enzyme with enhanced activity to depolymerize xylo-oligosaccharides. beta-1,4-xylosidase belongs to the GH43(1) family as classified in the Carbohydrate-Active Enzyme Database (CAZy). PsGH43(2) was found to be active only on xylose-based substrate, 4NPX(3), with maximum activity occurring at a pH 7 and 30 degrees C (K(m) 1.96 +/- 0.2 mM and V(max) 0.43 mM/min). The study also confirms the influence of Ca(2+) ions on enzymatic activity and thermal stability. Subsequently, native PsGH43 was crystallized at optimum conditions and the structure was determined at 2.5 A resolution. Crystallographic analysis revealed an asymmetric unit containing eight monomers and 16 calcium ions wherein a tetramer constituted the functional unit. Each monomer exhibits a characteristic GH43 N-terminal beta-propeller fold that serves as a catalytic domain accommodating one calcium ion in the centre, while the C-terminal beta-sandwich fold associated with the CBM6(4) family preserves another calcium ion. Our study reveals a novel tetrameric arrangement of beta-1,4-xylosidase which unravels its functional indispensability. This study opens newer avenues to engineer a potential enzyme for biofuel and bioethanol industry.


Authors: Vishwakarma, P., Sachdeva, E., Goyal, A., Ethayathulla, A.S., Das, U., Kaur, P.
Deciphering the structural and biochemical aspects of xylosidase from Pseudopedobacter saltans.,Vishwakarma P, Sachdeva E, Thakur A, Ethayathulla AS, Goyal A, Kaur P Int J Biol Macromol. 2024 Dec 19;291:139042. doi: 10.1016/j.ijbiomac.2024.139042. PMID:39708861<ref>PMID:39708861</ref>


Description: Structure of GH43 family enzyme, Xylan 1, 4 Beta-xylosidase from pseudopedobacter saltans
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Vishwakarma, P]]
<div class="pdbe-citations 8hcj" style="background-color:#fffaf0;"></div>
[[Category: Sachdeva, E]]
 
[[Category: Das, U]]
==See Also==
[[Category: Goyal, A]]
*[[Xylosidase 3D structures|Xylosidase 3D structures]]
[[Category: Ethayathulla, A.S]]
== References ==
[[Category: Kaur, P]]
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudopedobacter saltans DSM 12145]]
[[Category: Das U]]
[[Category: Ethayathulla AS]]
[[Category: Goyal A]]
[[Category: Kaur P]]
[[Category: Sachdeva E]]
[[Category: Vishwakarma P]]
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