8ps4: Difference between revisions

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'''Unreleased structure'''


The entry 8ps4 is ON HOLD  until Paper Publication
==Escherichia coli SduA complex==
<StructureSection load='8ps4' size='340' side='right'caption='[[8ps4]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ps4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_KTE10 Escherichia coli KTE10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PS4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ps4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ps4 OCA], [https://pdbe.org/8ps4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ps4 RCSB], [https://www.ebi.ac.uk/pdbsum/8ps4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ps4 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The detection of molecular patterns associated with invading pathogens is a hallmark of innate immune systems. Prokaryotes deploy sophisticated host defense mechanisms in innate anti-phage immunity. Shedu is a single-component defense system comprising a putative nuclease SduA. Here, we report cryoelectron microscopy (cryo-EM) structures of apo- and double-stranded DNA (dsDNA)-bound tetrameric SduA assemblies, revealing that the N-terminal domains of SduA form a clamp that recognizes free DNA ends. End binding positions the DNA over the PD-(D/E)XK nuclease domain, resulting in dsDNA nicking at a fixed distance from the 5' end. The end-directed DNA nicking activity of Shedu prevents propagation of linear DNA in vivo. Finally, we show that phages escape Shedu immunity by suppressing their recombination-dependent DNA replication pathway. Taken together, these results define the antiviral mechanism of Shedu systems, underlining the paradigm that recognition of pathogen-specific nucleic acid structures is a conserved feature of innate immunity across all domains of life.


Authors:  
DNA end sensing and cleavage by the Shedu anti-phage defense system.,Loeff L, Walter A, Rosalen GT, Jinek M Cell. 2024 Dec 30:S0092-8674(24)01346-1. doi: 10.1016/j.cell.2024.11.030. PMID:39742808<ref>PMID:39742808</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 8ps4" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli KTE10]]
[[Category: Large Structures]]
[[Category: Jinek M]]
[[Category: Loeff L]]
Retrieved from "https://proteopedia.org/8ps4"