9enw: Difference between revisions

Latest revision as of 21:44, 26 March 2025

Apo GES-1 beta-Lactamase

Structural highlights

9enw is a 2 chain structure with sequence from Klebsiella pneumoniae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.66Å
Ligands:	EPE
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BLAG1_KLEPN Extended-spectrum beta-lactamase (ESBL) which confers resistance to penicillins, as well as first, second, third and fourth-generation cephalosporins (PubMed:10681329, PubMed:12936982, PubMed:19656947, PubMed:20696873, PubMed:29507065). Has ceftazidime-hydrolyzing activity (PubMed:10681329, PubMed:12936982, PubMed:19656947, PubMed:20696873, PubMed:29507065). Inactive against the carbapenems, imipenem, meropenem, ertapenem and doripenem (PubMed:10681329, PubMed:19656947, PubMed:29507065). However, weak hydrolytic activity with respect to imipenem has also been reported (PubMed:20696873).^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Poirel L, Le Thomas I, Naas T, Karim A, Nordmann P. Biochemical sequence analyses of GES-1, a novel class A extended-spectrum beta-lactamase, and the class 1 integron In52 from Klebsiella pneumoniae. Antimicrob Agents Chemother. 2000 Mar;44(3):622-32. PMID:10681329 doi:10.1128/AAC.44.3.622-632.2000
↑ Correia M, Boavida F, Grosso F, Salgado MJ, Lito LM, Cristino JM, Mendo S, Duarte A. Molecular characterization of a new class 3 integron in Klebsiella pneumoniae. Antimicrob Agents Chemother. 2003 Sep;47(9):2838-43. PMID:12936982 doi:10.1128/AAC.47.9.2838-2843.2003
↑ Frase H, Shi Q, Testero SA, Mobashery S, Vakulenko SB. Mechanistic basis for the emergence of catalytic competence against carbapenem antibiotics by the GES family of beta-lactamases. J Biol Chem. 2009 Oct 23;284(43):29509-13. PMID:19656947 doi:10.1074/jbc.M109.011262
↑ Kotsakis SD, Miriagou V, Tzelepi E, Tzouvelekis LS. Comparative biochemical and computational study of the role of naturally occurring mutations at Ambler positions 104 and 170 in GES β-lactamases. Antimicrob Agents Chemother. 2010 Nov;54(11):4864-71. PMID:20696873 doi:10.1128/AAC.00771-10
↑ Piccirilli A, Mercuri PS, Galleni M, Aschi M, Matagne A, Amicosante G, Perilli M. P174E Substitution in GES-1 and GES-5 β-Lactamases Improves Catalytic Efficiency toward Carbapenems. Antimicrob Agents Chemother. 2018 Apr 26;62(5):e01851-17. PMID:29507065 doi:10.1128/AAC.01851-17

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Poirel L, Le Thomas I, Naas T, Karim A, Nordmann P. Biochemical sequence analyses of GES-1, a novel class A extended-spectrum beta-lactamase, and the class 1 integron In52 from Klebsiella pneumoniae. Antimicrob Agents Chemother. 2000 Mar;44(3):622-32. PMID:10681329 doi:10.1128/AAC.44.3.622-632.2000

[2] Correia M, Boavida F, Grosso F, Salgado MJ, Lito LM, Cristino JM, Mendo S, Duarte A. Molecular characterization of a new class 3 integron in Klebsiella pneumoniae. Antimicrob Agents Chemother. 2003 Sep;47(9):2838-43. PMID:12936982 doi:10.1128/AAC.47.9.2838-2843.2003

[3] Frase H, Shi Q, Testero SA, Mobashery S, Vakulenko SB. Mechanistic basis for the emergence of catalytic competence against carbapenem antibiotics by the GES family of beta-lactamases. J Biol Chem. 2009 Oct 23;284(43):29509-13. PMID:19656947 doi:10.1074/jbc.M109.011262

[4] Kotsakis SD, Miriagou V, Tzelepi E, Tzouvelekis LS. Comparative biochemical and computational study of the role of naturally occurring mutations at Ambler positions 104 and 170 in GES β-lactamases. Antimicrob Agents Chemother. 2010 Nov;54(11):4864-71. PMID:20696873 doi:10.1128/AAC.00771-10

[5] Piccirilli A, Mercuri PS, Galleni M, Aschi M, Matagne A, Amicosante G, Perilli M. P174E Substitution in GES-1 and GES-5 β-Lactamases Improves Catalytic Efficiency toward Carbapenems. Antimicrob Agents Chemother. 2018 Apr 26;62(5):e01851-17. PMID:29507065 doi:10.1128/AAC.01851-17

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9enw is ON HOLD  until Paper Publication
+==Apo GES-1 beta-Lactamase==
+<StructureSection load='9enw' size='340' side='right'caption='[[9enw]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
-Authors: Beer, M., Hinchliffe, P., Spencer, J.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9enw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9ENW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9ENW FirstGlance]. <br>
-Description: Apo GES-1 beta-Lactamase
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>
-[[Category: Spencer, J]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9enw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9enw OCA], [https://pdbe.org/9enw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9enw RCSB], [https://www.ebi.ac.uk/pdbsum/9enw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9enw ProSAT]</span></td></tr>
-[[Category: Hinchliffe, P]]
+</table>
-[[Category: Beer, M]]
+== Function ==
+[https://www.uniprot.org/uniprot/BLAG1_KLEPN BLAG1_KLEPN] Extended-spectrum beta-lactamase (ESBL) which confers resistance to penicillins, as well as first, second, third and fourth-generation cephalosporins (PubMed:10681329, PubMed:12936982, PubMed:19656947, PubMed:20696873, PubMed:29507065). Has ceftazidime-hydrolyzing activity (PubMed:10681329, PubMed:12936982, PubMed:19656947, PubMed:20696873, PubMed:29507065). Inactive against the carbapenems, imipenem, meropenem, ertapenem and doripenem (PubMed:10681329, PubMed:19656947, PubMed:29507065). However, weak hydrolytic activity with respect to imipenem has also been reported (PubMed:20696873).<ref>PMID:10681329</ref> <ref>PMID:12936982</ref> <ref>PMID:19656947</ref> <ref>PMID:20696873</ref> <ref>PMID:29507065</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Klebsiella pneumoniae]]
+[[Category: Large Structures]]
+[[Category: Beer M]]
+[[Category: Hinchliffe P]]
+[[Category: Spencer J]]

9enw: Difference between revisions

Latest revision as of 21:44, 26 March 2025

Apo GES-1 beta-Lactamase

Structural highlights

Function

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