8axj

Crystal structure of the N-terminal domain of Trypanosoma brucei CFAP410

spinqualitylabelsall models 8axj, resolution 1.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8axj is a 1 chain structure with sequence from Trypanosoma brucei brucei TREU927. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1Å Ligands: GOL, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q38DI2_TRYB2 Publication Abstract from PubMed Cilia and flagella associated protein 410 (CFAP410) is a protein localized at the basal body of cilia/flagella and plays essential roles in ciliogenesis. Multiple single amino acid mutations in CFAP410 have been identified in patients. However, the molecular mechanism for how the mutations cause these disorders remains poorly understood due to a lack of high-resolution structures of the protein. Our studies demonstrate that CFAP410 adopts a bimodular architecture. We have previously reported our structural studies on the C-terminal domain (CTD) of CFAP410 from various organisms. Here we report a 1.0-A resolution crystal structure of the N-terminal domain (NTD) of Trypanosoma brucei CFAP410. We further examined how the disease-causing mutations in this domain may affect the folding and structural stability of CFAP410. Our results suggest that the single-residue mutations in the CFAP410-NTD cause human diseases by destabilizing the structure that subsequently disrupts its interaction with other partners. CFAP410 has a bimodular architecture with a conserved surface patch on its N-terminal leucine-rich repeat motif for binding interaction partners.,Stadler A, Gabriel HB, De Liz LV, Alonso-Gil S, Deng X, Crickley R, Korbula K, Mikolaskova B, Vaughan S, Huang K, Zagrovic B, Sunter JD, Dong G Front Cell Dev Biol. 2025 Feb 13;13:1507470. doi: 10.3389/fcell.2025.1507470. , eCollection 2025. PMID:40018707 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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