6w98: Difference between revisions

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<StructureSection load='6w98' size='340' side='right'caption='[[6w98]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
<StructureSection load='6w98' size='340' side='right'caption='[[6w98]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6w98]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"mycobacterium_abscessus_subsp._abscessus"_leao_et_al._2009 "mycobacterium abscessus subsp. abscessus" leao et al. 2009] and [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W98 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W98 FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W98 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W98 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6OU:[(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl]+(~{Z})-octadec-9-enoate'>6OU</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAMEA2161603_00457 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1185650 "Mycobacterium abscessus subsp. abscessus" Leao et al. 2009])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6OU:[(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl]+(~{Z})-octadec-9-enoate'>6OU</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w98 OCA], [https://pdbe.org/6w98 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w98 RCSB], [https://www.ebi.ac.uk/pdbsum/6w98 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w98 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w98 OCA], [https://pdbe.org/6w98 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w98 RCSB], [https://www.ebi.ac.uk/pdbsum/6w98 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w98 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/ACP_ECOLI ACP_ECOLI]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mycobacterium tuberculosis causes tuberculosis, a disease that kills over 1 million people each year. Its cell envelope is a common antibiotic target and has a unique structure due, in part, to two lipidated polysaccharides-arabinogalactan and lipoarabinomannan. Arabinofuranosyltransferase D (AftD) is an essential enzyme involved in assembling these glycolipids. We present the 2.9-A resolution structure of M. abscessus AftD, determined by single-particle cryo-electron microscopy. AftD has a conserved GT-C glycosyltransferase fold and three carbohydrate-binding modules. Glycan array analysis shows that AftD binds complex arabinose glycans. Additionally, AftD is non-covalently complexed with an acyl carrier protein (ACP). 3.4- and 3.5-A structures of a mutant with impaired ACP binding reveal a conformational change, suggesting that ACP may regulate AftD function. Mutagenesis experiments using a conditional knockout constructed in M. smegmatis confirm the essentiality of the putative active site and the ACP binding for AftD function.
Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria.,Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosario AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalao MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F Mol Cell. 2020 May 5. pii: S1097-2765(20)30256-2. doi:, 10.1016/j.molcel.2020.04.014. PMID:32386575<ref>PMID:32386575</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6w98" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Acyl carrier protein 3D structures|Acyl carrier protein 3D structures]]
*[[Acyl carrier protein 3D structures|Acyl carrier protein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Mycobacterium abscessus subsp. abscessus leao et al. 2009]]
[[Category: Ecoli]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Archer, M]]
[[Category: Archer M]]
[[Category: Athayde, D]]
[[Category: Athayde D]]
[[Category: Brunton, R]]
[[Category: Brunton R]]
[[Category: Carragher, B]]
[[Category: Carragher B]]
[[Category: Catalao, M J]]
[[Category: Catalao MJ]]
[[Category: Clarke, O B]]
[[Category: Clarke OB]]
[[Category: Dandey, V P]]
[[Category: Dandey VP]]
[[Category: Giacometti, S I]]
[[Category: Giacometti SI]]
[[Category: Kloss, B]]
[[Category: Kloss B]]
[[Category: Lowary, T L]]
[[Category: Lowary TL]]
[[Category: Mancia, F]]
[[Category: Mancia F]]
[[Category: Niederweis, M]]
[[Category: Niederweis M]]
[[Category: Pimentel, M]]
[[Category: Pimentel M]]
[[Category: Potter, C S]]
[[Category: Potter CS]]
[[Category: Raczkowski, A M]]
[[Category: Raczkowski AM]]
[[Category: Rodrigues, J]]
[[Category: Rodrigues J]]
[[Category: Rosario, A L]]
[[Category: Rosario AL]]
[[Category: Tan, Y Z]]
[[Category: Tan YZ]]
[[Category: Wei, H]]
[[Category: Wei H]]
[[Category: Zhang, L]]
[[Category: Zhang L]]
[[Category: Zheng, R B]]
[[Category: Zheng RB]]
[[Category: Acyl carrier protein]]
[[Category: Arabinofuranose]]
[[Category: Glycosyltransferase]]
[[Category: Lipoarabinomannan]]
[[Category: Lipomannan]]
[[Category: Membrane protein]]
[[Category: Nanodisc]]
[[Category: Single-particle cryo-electron microscopy]]

Latest revision as of 09:49, 2 April 2025

Single-Particle Cryo-EM Structure of Arabinofuranosyltransferase AftD from Mycobacteria

6w98, resolution 2.90Å

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