|
|
| Line 3: |
Line 3: |
| <StructureSection load='6yl3' size='340' side='right'caption='[[6yl3]], [[Resolution|resolution]] 1.98Å' scene=''> | | <StructureSection load='6yl3' size='340' side='right'caption='[[6yl3]], [[Resolution|resolution]] 1.98Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6yl3]] is a 36 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica_w22703 Yersinia enterocolitica w22703]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YL3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YL3 FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YL3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YL3 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 1.98Å</td></tr> |
| <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] </span></td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yl3 OCA], [https://pdbe.org/6yl3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yl3 RCSB], [https://www.ebi.ac.uk/pdbsum/6yl3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yl3 ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yl3 OCA], [http://pdbe.org/6yl3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yl3 RCSB], [http://www.ebi.ac.uk/pdbsum/6yl3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yl3 ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| Urease converts urea into ammonia and carbon dioxide and makes urea available as a nitrogen source for all forms of life except animals. In human bacterial pathogens, ureases also aid in the invasion of acidic environments such as the stomach by raising the surrounding pH. Here, we report the structure of urease from the pathogen Yersinia enterocolitica at 2 A resolution from cryo-electron microscopy. Y. enterocolitica urease is a dodecameric assembly of a trimer of three protein chains, ureA, ureB and ureC. The high data quality enables detailed visualization of the urease bimetal active site and of the impact of radiation damage. The obtained structure is of sufficient quality to support drug development efforts.
| |
|
| |
|
| High-resolution cryo-EM structure of urease from the pathogen Yersinia enterocolitica.,Righetto RD, Anton L, Adaixo R, Jakob RP, Zivanov J, Mahi MA, Ringler P, Schwede T, Maier T, Stahlberg H Nat Commun. 2020 Oct 9;11(1):5101. doi: 10.1038/s41467-020-18870-2. PMID:33037208<ref>PMID:33037208</ref>
| | ==See Also== |
| | | *[[Urease 3D structures|Urease 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 6yl3" style="background-color:#fffaf0;"></div>
| |
| == References == | |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Urease]]
| | [[Category: Adaixo R]] |
| [[Category: Yersinia enterocolitica w22703]]
| | [[Category: Anton L]] |
| [[Category: Adaixo, R]] | | [[Category: Jakob R]] |
| [[Category: Anton, L]] | | [[Category: Mahi MA]] |
| [[Category: Jakob, R]] | | [[Category: Maier T]] |
| [[Category: Mahi, M A]] | | [[Category: Righetto RD]] |
| [[Category: Maier, T]] | | [[Category: Ringler P]] |
| [[Category: Righetto, R D]] | | [[Category: Schwede T]] |
| [[Category: Ringler, P]] | | [[Category: Stahlberg H]] |
| [[Category: Schwede, T]] | | [[Category: Zivanov J]] |
| [[Category: Stahlberg, H]] | |
| [[Category: Zivanov, J]] | |
| [[Category: Enzyme]]
| |
| [[Category: Metal binding protein]]
| |
| [[Category: Metalloenzyme]]
| |
| [[Category: Nickel]]
| |
| [[Category: Pathogen]]
| |
