Sandbox Reserved 1844: Difference between revisions
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As the PET substrate binds to the enzyme, its carbonyl bond | As the PET substrate binds to the enzyme, its carbonyl bond | ||
attached to the first benzene ring must be harbored close to | attached to the first benzene ring must be harbored close to serine in | ||
the catalytic triad (Fig. 2). Serine gets polarized by histidine, which is | the <scene name='10/1075247/Active_site_residues/1'>catalytic triad Location</scene> (Fig. 2). Serine gets polarized by histidine, which is | ||
then stabilized by aspartic acid. Polarized | then stabilized by aspartic acid. Polarized serine will attack the | ||
carbonyl bond (C-O) of the polyester, resulting in a tetrahedral in- | carbonyl bond (C-O) of the polyester, resulting in a tetrahedral in- | ||
termediate, which is then stabilized by the oxyanion hole. The | termediate, which is then stabilized by the oxyanion hole. The hydrolysis procedure is completed by a second nucleophilic attack | ||
mediated through a water molecule "PEThydrolase-insilico-engineering". | |||
mediated through a water molecule "PEThydrolase-insilico-engineering" | |||
[[Image:Mechanism_(1).jpeg|1100 px]] | [[Image:Mechanism_(1).jpeg|1100 px]] | ||
