9k3u: Difference between revisions

Latest revision as of 11:28, 16 April 2025

Human RNA Polymerase III de novo transcribing complex 5 (TC5)

Structural highlights

9k3u is a 10 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3Å
Ligands:	GTP, MG, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

RPAC2_HUMAN The disease is caused by variants affecting the gene represented in this entry.

Function

RPAC2_HUMAN DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and short non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs, respectively.[UniProtKB:P28000]^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Canella D, Praz V, Reina JH, Cousin P, Hernandez N. Defining the RNA polymerase III transcriptome: Genome-wide localization of the RNA polymerase III transcription machinery in human cells. Genome Res. 2010 Jun;20(6):710-21. PMID:20413673 doi:10.1101/gr.101337.109
↑ Zhao D, Liu W, Chen K, Wu Z, Yang H, Xu Y. Structure of the human RNA polymerase I elongation complex. Cell Discov. 2021 Oct 20;7(1):97. PMID:34671025 doi:10.1038/s41421-021-00335-5
↑ Misiaszek AD, Girbig M, Grötsch H, Baudin F, Murciano B, Lafita A, Müller CW. Cryo-EM structures of human RNA polymerase I. Nat Struct Mol Biol. 2021 Dec;28(12):997-1008. PMID:34887565 doi:10.1038/s41594-021-00693-4
↑ Van Bortle K, Marciano DP, Liu Q, Chou T, Lipchik AM, Gollapudi S, Geller BS, Monte E, Kamakaka RT, Snyder MP. A cancer-associated RNA polymerase III identity drives robust transcription and expression of snaR-A noncoding RNA. Nat Commun. 2022 May 30;13(1):3007. PMID:35637192 doi:10.1038/s41467-022-30323-6
↑ Daiß JL, Pilsl M, Straub K, Bleckmann A, Höcherl M, Heiss FB, Abascal-Palacios G, Ramsay EP, Tlučková K, Mars JC, Fürtges T, Bruckmann A, Rudack T, Bernecky C, Lamour V, Panov K, Vannini A, Moss T, Engel C. The human RNA polymerase I structure reveals an HMG-like docking domain specific to metazoans. Life Sci Alliance. 2022 Sep 1;5(11):e202201568. PMID:36271492 doi:10.26508/lsa.202201568

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OCA

[1] Canella D, Praz V, Reina JH, Cousin P, Hernandez N. Defining the RNA polymerase III transcriptome: Genome-wide localization of the RNA polymerase III transcription machinery in human cells. Genome Res. 2010 Jun;20(6):710-21. PMID:20413673 doi:10.1101/gr.101337.109

[2] Zhao D, Liu W, Chen K, Wu Z, Yang H, Xu Y. Structure of the human RNA polymerase I elongation complex. Cell Discov. 2021 Oct 20;7(1):97. PMID:34671025 doi:10.1038/s41421-021-00335-5

[3] Misiaszek AD, Girbig M, Grötsch H, Baudin F, Murciano B, Lafita A, Müller CW. Cryo-EM structures of human RNA polymerase I. Nat Struct Mol Biol. 2021 Dec;28(12):997-1008. PMID:34887565 doi:10.1038/s41594-021-00693-4

[4] Van Bortle K, Marciano DP, Liu Q, Chou T, Lipchik AM, Gollapudi S, Geller BS, Monte E, Kamakaka RT, Snyder MP. A cancer-associated RNA polymerase III identity drives robust transcription and expression of snaR-A noncoding RNA. Nat Commun. 2022 May 30;13(1):3007. PMID:35637192 doi:10.1038/s41467-022-30323-6

[5] Daiß JL, Pilsl M, Straub K, Bleckmann A, Höcherl M, Heiss FB, Abascal-Palacios G, Ramsay EP, Tlučková K, Mars JC, Fürtges T, Bruckmann A, Rudack T, Bernecky C, Lamour V, Panov K, Vannini A, Moss T, Engel C. The human RNA polymerase I structure reveals an HMG-like docking domain specific to metazoans. Life Sci Alliance. 2022 Sep 1;5(11):e202201568. PMID:36271492 doi:10.26508/lsa.202201568

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9k3u is ON HOLD  until Paper Publication
+==Human RNA Polymerase III de novo transcribing complex 5 (TC5)==
+<StructureSection load='9k3u' size='340' side='right'caption='[[9k3u]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9k3u]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9K3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9K3U FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9k3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9k3u OCA], [https://pdbe.org/9k3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9k3u RCSB], [https://www.ebi.ac.uk/pdbsum/9k3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9k3u ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/RPAC2_HUMAN RPAC2_HUMAN] The disease is caused by variants affecting the gene represented in this entry.
+== Function ==
+[https://www.uniprot.org/uniprot/RPAC2_HUMAN RPAC2_HUMAN] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and short non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs, respectively.[UniProtKB:P28000]<ref>PMID:20413673</ref> <ref>PMID:34671025</ref> <ref>PMID:34887565</ref> <ref>PMID:35637192</ref> <ref>PMID:36271492</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Chen X]]
+[[Category: Jin Q]]
+[[Category: Ren Y]]
+[[Category: Wang Q]]
+[[Category: Xu Y]]

9k3u: Difference between revisions

Latest revision as of 11:28, 16 April 2025

Human RNA Polymerase III de novo transcribing complex 5 (TC5)

Structural highlights

Disease

Function

References

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