Sandbox Reserved 1845: Difference between revisions
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=== Tyr 127 === | === Tyr 127 === | ||
The mutation of Tyr to Gly at <scene name='10/1075247/Y127/4'>Y127</scene> also increases the protein's thermostability. The mutant melting point is increased to 87.0°C. Tyr has a bulky, rigid aromatic side chain that can cause structural strain. Gly is the smallest amino acid and lacks a side chain, so it provides greater flexibility to the protein. The <scene name='10/1075247/Y127g/2'>Y127G</scene> mutation reduces [https://en.wikipedia.org/wiki/Steric_effects#:~:text=Steric%20hindrance%20is%20the%20slowing,as%20slowing%20unwanted%20side%2Dreactions. steric hindrance] and relieves strain in the protein structure. By increasing flexibility, the Y127G mutation helps the protein maintain its folded structure under heat stress.<ref name="Tournier"/> | The mutation of Tyr to Gly at <scene name='10/1075247/Y127/4'>Y127</scene> also increases the protein's thermostability. The mutant melting point is increased to 87.0°C. Tyr has a bulky, rigid aromatic side chain that can cause structural strain, <scene name='10/1075247/Y127_spacefill/1'>Y127 representation</scene>. Gly is the smallest amino acid and lacks a side chain, so it provides greater flexibility to the protein. The <scene name='10/1075247/Y127g/2'>Y127G</scene> mutation reduces [https://en.wikipedia.org/wiki/Steric_effects#:~:text=Steric%20hindrance%20is%20the%20slowing,as%20slowing%20unwanted%20side%2Dreactions. steric hindrance] and relieves strain in the protein structure. By increasing flexibility, the Y127G mutation helps the protein maintain its folded structure under heat stress.<ref name="Tournier"/> | ||
=== Ser 283 & Asp 238 === | === Ser 283 & Asp 238 === | ||
