1vm1: Difference between revisions
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'''STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM''' | '''STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM''' | ||
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==About this Structure== | ==About this Structure== | ||
1VM1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. This structure supersedes the now removed PDB entry | 1VM1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1g56 1g56]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VM1 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Nukaga, M.]] | [[Category: Nukaga, M.]] | ||
[[Category: Nukaga, Y.]] | [[Category: Nukaga, Y.]] | ||
[[Category: | [[Category: Beta-lactam hydrolase]] | ||
[[Category: | [[Category: Beta-lactamase]] | ||
[[Category: | [[Category: Detergent binding]] | ||
[[Category: | [[Category: Inhibitor design]] | ||
[[Category: | [[Category: Penicillinase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:42:11 2008'' | |||
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Revision as of 09:42, 3 May 2008
STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM
Overview
Two species resulting from the reaction of the SHV-1 class A beta-lactamase with the sulfone inhibitor tazobactam have been trapped at 100 K and mapped by X-ray crystallography at 2.0 A resolution. An acyclic form of tazobactam is covalently bonded to the catalytic Ser70 side chain, and a second species, a five-atom vinyl carboxylic acid fragment of tazobactam, is bonded to Ser130. It is proposed that the electron density map of the crystal is a composite picture of two complexes, each with only a single bound species. It is estimated that the two complexes exist in the crystal in approximately equal populations. Results are discussed in relation to the mechanism-based inhibition of class A beta-lactamases by the similar inhibitors sulbactam and clavulanic acid.
About this Structure
1VM1 is a Single protein structure of sequence from Klebsiella pneumoniae. This structure supersedes the now removed PDB entry 1g56. Full crystallographic information is available from OCA.
Reference
Inhibition of the SHV-1 beta-lactamase by sulfones: crystallographic observation of two reaction intermediates with tazobactam., Kuzin AP, Nukaga M, Nukaga Y, Hujer A, Bonomo RA, Knox JR, Biochemistry. 2001 Feb 13;40(6):1861-6. PMID:11327849 Page seeded by OCA on Sat May 3 12:42:11 2008
