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4Q7Q is a homodimeric protein complex that originates from the bacterial species Chitinophaga Pinensis and has a mass of 58.5 kDa. It is a member of the SGNH Hydrolase Superfamily with structural and sequential similarities to esterases and lipases. Current evidence suggests it causes the hydrolysis of esters and/or acetyl groups on lipids/lipid-like molecules via a serine protease-like active site. | 4Q7Q is a homodimeric protein complex that originates from the bacterial species Chitinophaga Pinensis and has a mass of 58.5 kDa. It is a member of the SGNH Hydrolase Superfamily with structural and sequential similarities to esterases and lipases. Current evidence suggests it causes the hydrolysis of esters and/or acetyl groups on lipids/lipid-like molecules via a serine protease-like active site. | ||
<StructureSection load='4Q7Q' size=' | <StructureSection load='4Q7Q' size='300' side='right' caption='3D Representation of 4Q7Q's structure' scene=''> | ||
== Overview == | == Overview == | ||
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=== General Structure and Origins === | === General Structure and Origins === | ||
4Q7Q exists as a homodimer Quaternary structure.<ref name="Sequence">4Q7Q. Protein Database, 2014.</ref> Analyzing primary and Quaternary structures of 4Q7Q with SPRITE and Chimera revealed two chains identical in both shape and sequence<ref name="SPRITE">Nadzirin, N.; Gardiner, E.; Willett, P.; Artymiuk, P. J.; Firdaus-Raih, M. 2012. SPRITE and ASSAM: web servers for side chain 3D-motif searching in protein structures. Nucleic Acids Res., 40(Web Server Issue), W380-6.</ref> Each chain is 266 residues long, and the entire complex has a molecular weight of approximately 58.5 kDa.<ref name="Sequence" />. | 4Q7Q exists as a homodimer Quaternary structure.<ref name="Sequence">4Q7Q. Protein Database, 2014. https://www.rcsb.org/structure/4Q7Q</ref> Analyzing primary and Quaternary structures of 4Q7Q with SPRITE and Chimera revealed two chains identical in both shape and sequence<ref name="SPRITE">Nadzirin, N.; Gardiner, E.; Willett, P.; Artymiuk, P. J.; Firdaus-Raih, M. 2012. SPRITE and ASSAM: web servers for side chain 3D-motif searching in protein structures. Nucleic Acids Res., 40(Web Server Issue), W380-6.</ref> Each chain is 266 residues long, and the entire complex has a molecular weight of approximately 58.5 kDa.<ref name="Sequence" />. | ||
[[Image:4Q7QABSequence.png| | [[Image:4Q7QABSequence.png|400px|left|thumb| Primary Sequences of the A and B chains of 4Q7Q.<ref name="SPRITE" />.]] | ||
4Q7Q proteins primarily appear in bacterial species.<ref name="Origin">Rio, T. G. D.; et al. Complete genome sequence of Chitinophaga pinensis type strain (UQM 2034). Stand. Genomic. Sci., 2010, 2(1), 87-95. https://pmc.ncbi.nlm.nih.gov/articles/PMC3035255/</ref>. Research reveals how nearly every enzyme with similar sequencing to 4Q7Q is found in bacterium, with a slight exception to eukaryotes.<ref name="SGNH">SGNH hydrolase superfamily. InterPro, 2017. https://www.ebi.ac.uk/interpro/entry/InterPro/IPR036514/</ref> Additionally, the PDB entry for 4Q7Q notes how it potentially can be found in Chitinophaga pinensis, a gram-negative bacterial species which can degrade chitin.<ref name="Origin" />. <ref name="GDSL">Rio, T. G. D.; et al. Complete genome sequence of Chitinophaga pinensis type strain (UQM 2034). Stand. Genomic. Sci., 2010, 2(1), 87-95. https://pmc.ncbi.nlm.nih.gov/articles/PMC3035255/ </ref>. | 4Q7Q proteins primarily appear in bacterial species.<ref name="Origin">Rio, T. G. D.; et al. Complete genome sequence of Chitinophaga pinensis type strain (UQM 2034). Stand. Genomic. Sci., 2010, 2(1), 87-95. https://pmc.ncbi.nlm.nih.gov/articles/PMC3035255/</ref>. Research reveals how nearly every enzyme with similar sequencing to 4Q7Q is found in bacterium, with a slight exception to eukaryotes.<ref name="SGNH">SGNH hydrolase superfamily. InterPro, 2017. https://www.ebi.ac.uk/interpro/entry/InterPro/IPR036514/</ref> Additionally, the PDB entry for 4Q7Q notes how it potentially can be found in Chitinophaga pinensis, a gram-negative bacterial species which can degrade chitin.<ref name="Origin" />. <ref name="GDSL">Rio, T. G. D.; et al. Complete genome sequence of Chitinophaga pinensis type strain (UQM 2034). Stand. Genomic. Sci., 2010, 2(1), 87-95. https://pmc.ncbi.nlm.nih.gov/articles/PMC3035255/ </ref>. | ||
