Sandbox323: Difference between revisions
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[[Image:Ice_bath_basic_conditions_with_salt.jpg|800px|left|]] | [[Image:Ice_bath_basic_conditions_with_salt.jpg|800px|left|]] | ||
50uL of purified protein from the first elution was added to 3mL of cold 1mg/mL PNPA. Solid sodium chloride was added to achieve a final concentration of 5mM NaCl. The absorbance peak was measured every 60 seconds for 30 minutes. Peak maximum was located at 427.8nm. The sample incubated in an ice bath between measurements. No discernible relationship between time and absorbance was noted. pH of the solution was 10.60. The instrument to measure pH was Vernier Lab Quest 3 with Tris-compatible flat pH sensor calibrated with two points. | |||
== References == | == References == | ||
<references/> | <references/> | ||
