Sandbox323: Difference between revisions
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== Experimental Data == | == Experimental Data == | ||
=== | === Bacterium === | ||
=== Hydrolysis of Substrate === | Chitinophaga pinensis is an obligate aerobe, spore-forming, psychrophilic bacterium that was isolated from pine litter. | ||
=== Substrate and Hydrolysis of Substrate === | |||
p-nitrophenyl acetate (PNPA) is an aromatic compound with an ester linkage. In the presence of an esterase, the compound is susceptible to cleavage. The products are acetate and p-nitrophenolate anion (PNP). PNP is yellow in color so reaction progress can be monitored by measuring the absorption peak at 405nm using a spectrophotometer. | |||
=== Spectrophotometry === | === Spectrophotometry === | ||
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The cold 4 degrees C reaction was changed to room temperature 20 degrees C. All other conditions remained constant to evaluate the effect of temperature on enzyme. | The cold 4 degrees C reaction was changed to room temperature 20 degrees C. All other conditions remained constant to evaluate the effect of temperature on enzyme. Temperature increase negatively affects enzyme performance. Considering the | ||
[[Image:Roomtempconcentrationtime.png|700px|left|thumb|Enzyme Activity at neutral pH at | [[Image:Roomtempconcentrationtime.png|700px|left|thumb|Enzyme Activity at neutral pH at room temperature with Chloride cofactor]] | ||
[[Image:Sigfigroomtempenzymeactivity.png|700px|left|thumb|Enzyme Activity at neutral pH at room temperature with Chloride cofactor ]] | [[Image:Sigfigroomtempenzymeactivity.png|700px|left|thumb|Enzyme Activity at neutral pH at room temperature with Chloride cofactor ]] | ||
