Sandbox323: Difference between revisions
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The cold 4 degrees C reaction was changed to room temperature 20 degrees C. All other conditions remained constant to evaluate the effect of temperature on enzyme. Temperature increase negatively affects enzyme performance. Considering the cold loving nature of Chitinophaga pinensis, the enzyme being more active at a lower temperature is a reasonable conclusion. | The cold 4 degrees C reaction was changed to room temperature 20 degrees C. All other conditions remained constant to evaluate the effect of temperature on enzyme. Temperature increase negatively affects enzyme performance. Considering the cold loving nature of Chitinophaga pinensis, the enzyme being more active at a lower temperature is a reasonable conclusion. | ||
[[Image:Roomtempconcentrationtime.png| | [[Image:Roomtempconcentrationtime.png|900px|right|thumb|Enzyme Activity at neutral pH at room temperature with Chloride cofactor.]] | ||
[[Image:Sigfigroomtempenzymeactivity.png| | [[Image:Sigfigroomtempenzymeactivity.png|900px|right|thumb|Enzyme Activity at neutral pH at room temperature with Chloride cofactor.]] | ||
[[Image:Enzyme units percentage increase.png| | [[Image:Enzyme units percentage increase.png|900px|left|thumb|4 degrees C yields 42.2% increase in Units of Enzyme Activity цmol/minute.]] | ||
=== Conclusion === | === Conclusion === | ||
