Sandbox2O14: Difference between revisions
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== Protein Extraction == | == Protein Extraction == | ||
The protein was grown inside of ''E.coli'' with a His-tag for resistance to the antibiotic, Ampicillin. Once the protein was grown, the protein solution was centrifuged and sonicated multiple times to extract the expressed protein. For purification, the protein solution was ran through a Ni-NTA column gathering approximately | The protein was grown inside of ''E.coli'' with a His-tag for resistance to the antibiotic, Ampicillin. Once the protein was grown, the protein solution was centrifuged and sonicated multiple times to extract the expressed protein. For purification, the protein solution was ran through a Ni-NTA column gathering approximately 5 mL of protein elutions in total. | ||
== Figure 4 == | == Figure 4 == | ||
