Sandbox Reserved 1849: Difference between revisions

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Similarly, ACE2 forms a hydrogen bond with the Lys417 from its Asp30 residue. LCB1, however, forms two hydrogen bonds with both the Lys417 and Arg403 of the spike protein from its Asp30 residue. This is a very strong interaction, further contributing to the high affinity of LCB1 to the spike protein<ref name="Longxing">PMID:32907861</ref>.

==Implications==

~~===Potency of the minibinders===~~

Examining the IC50 values of the various mini binders gives quantitative data to the effectiveness of the proteins in preventing an immune response. The highest IC50 was AHB2 (15.5 nM), followed by LCB3 (40.1 pM) LCB1 (23.5 pM) <ref name="Longxing">PMID:32907861</ref>. The higher IC50 indicates a larger concentration of mini binder required to inhibit the biological process. Both LCB1 and LCB3 proved to be significantly more effective than AHB2, LCB1 and LCB3 were within 3-fold of the most potent anti-Spike monoclonal antibodies described to date <ref name="Longxing">PMID:32907861</ref>.

===Results from mice study===

The effectiveness of the most potent minibinder was examined in mice. LCB1 was administered to the mice via nasal delivery. As expected, compared to control mini protein, the LCB1 was significantly more effective at reducing the viral burden, diminishing the immune cell infiltration, and inflammation <ref name="Case">PMID:34192518</ref>. The virus was not detected in the lungs 4-7 days post-infection, and the spleen, heart, and brain had viral RNA at very low concentrations <ref name="Case">PMID:34192518</ref>.

Sandbox Reserved 1849: Difference between revisions

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@@ Line 56: / Line 56: @@
 Similarly, ACE2 forms a hydrogen bond with the Lys417 from its Asp30 residue. LCB1, however, forms two hydrogen bonds with both the Lys417 and Arg403 of the spike protein from its Asp30 residue. This is a very strong interaction, further contributing to the high affinity of LCB1 to the spike protein<ref name="Longxing">PMID:32907861</ref>.
 ==Implications==
-===Potency of the minibinders===
-Examining the IC<sub>50</sub> values of the various mini binders gives quantitative data to the effectiveness of the proteins in preventing an immune response. The highest IC<sub>50</sub> was AHB2 (15.5 nM), followed by LCB3 (40.1 pM) LCB1 (23.5 pM) <ref name="Longxing">PMID:32907861</ref>. The higher IC<sub>50</sub> indicates a larger concentration of mini binder required to inhibit the biological process. Both LCB1 and LCB3 proved to be significantly more effective than AHB2,  LCB1 and LCB3 were within 3-fold of the most potent anti-Spike monoclonal antibodies described to date <ref name="Longxing">PMID:32907861</ref>.
 ===Results from mice study===
 The effectiveness of the most potent minibinder was examined in mice. LCB1 was administered to the mice via nasal delivery. As expected, compared to control mini protein, the LCB1 was significantly more effective at reducing the viral burden, diminishing the immune cell infiltration, and inflammation <ref name="Case">PMID:34192518</ref>. The virus was not detected in the lungs 4-7 days post-infection, and the spleen, heart, and brain had viral RNA at very low concentrations <ref name="Case">PMID:34192518</ref>.