9hq6: Difference between revisions

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'''Unreleased structure'''


The entry 9hq6 is ON HOLD  until Paper Publication
==Structural insights in the HuHf@gold-monocarbene adduct: aurophilicity revealed in a biological context==
<StructureSection load='9hq6' size='340' side='right'caption='[[9hq6]], [[Resolution|resolution]] 1.51&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9hq6]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9HQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9HQ6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 1.51&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene>, <scene name='pdbligand=BM0:1-BUTYL-3-METHYL-1H-IMIDAZOL-3-IUM'>BM0</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9hq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9hq6 OCA], [https://pdbe.org/9hq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9hq6 RCSB], [https://www.ebi.ac.uk/pdbsum/9hq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9hq6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FRIH_HUMAN FRIH_HUMAN] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Human H ferritin (HuHf) has excellent potential as a nanocarrier for the selective delivery of anticancer metal-based drugs to tumor cells. Here, we addressed the interaction of the gold monocarbene compound Au(NHC)Cl with HuHf by electrospray ionization-mass spectrometry (ESI-MS) measurements, which provide the metalation state of the protein subunits and demonstrate the involvement of protein cysteines in gold binding. The adduct between Au(NHC)Cl and HuHf was studied by cryo-EM measurements, resulting in a high-resolution 3D density map at 1.51 A. The cryo-EM structure shows a novel tetranuclear gold(I) cluster, located in a surface pocket of each subunit where it is bound to Cys90 and Cys102. The short inter-metal distances are diagnostic of the occurrence of aurophilic interactions. The present work demonstrates the usefulness of cryo-EM to investigate the interactions between metal-based drugs and their protein targets/carriers, also leveraging the strong signal of transition metal ions.


Authors: Cosottini, L., Giachetti, A., Guerri, A., Martinez-Castillo, A., Geri, A., Zineddu, S., Abrescia, N.G.A., Messori, L., Turano, P., Rosato, A.
Structural Insight Into a Human H Ferritin@Gold-Monocarbene Adduct: Aurophilicity Revealed in a Biological Context.,Cosottini L, Giachetti A, Guerri A, Martinez-Castillo A, Geri A, Zineddu S, Abrescia NGA, Messori L, Turano P, Rosato A Angew Chem Int Ed Engl. 2025 Apr 18:e202503778. doi: 10.1002/anie.202503778. PMID:40249912<ref>PMID:40249912</ref>


Description: Structural insights in the HuHf@gold-monocarbene adduct: aurophilicity revealed in a biological context
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Abrescia, N.G.A]]
<div class="pdbe-citations 9hq6" style="background-color:#fffaf0;"></div>
[[Category: Messori, L]]
== References ==
[[Category: Guerri, A]]
<references/>
[[Category: Cosottini, L]]
__TOC__
[[Category: Turano, P]]
</StructureSection>
[[Category: Rosato, A]]
[[Category: Homo sapiens]]
[[Category: Giachetti, A]]
[[Category: Large Structures]]
[[Category: Zineddu, S]]
[[Category: Abrescia NGA]]
[[Category: Martinez-Castillo, A]]
[[Category: Cosottini L]]
[[Category: Geri, A]]
[[Category: Geri A]]
[[Category: Giachetti A]]
[[Category: Guerri A]]
[[Category: Martinez-Castillo A]]
[[Category: Messori L]]
[[Category: Rosato A]]
[[Category: Turano P]]
[[Category: Zineddu S]]
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