9lpb: Difference between revisions

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'''Unreleased structure'''


The entry 9lpb is ON HOLD  until Paper Publication
==Terpene cyclase AriE mutant-D128A==
<StructureSection load='9lpb' size='340' side='right'caption='[[9lpb]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9lpb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Amycolatopsis_arida Amycolatopsis arida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LPB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LPB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9lpb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9lpb OCA], [https://pdbe.org/9lpb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9lpb RCSB], [https://www.ebi.ac.uk/pdbsum/9lpb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9lpb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1I5P1T3_9PSEU A0A1I5P1T3_9PSEU]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Eunicellane diterpenoids, with over 360 known examples characterized by a cis- or trans-6/10-bicyclic carbon skeleton, exhibit diverse biological activities. To date, five eunicellane cyclases (ECs) have been reported: cis-ECs (Bnd4, CpDTS2 and EcTPS1) and trans-ECs (AlbS and MicA), yet the structural basis and molecular mechanisms governing their distinct cis- or trans-cyclization modes remain unsolved. Here, we present the crystal structure of a cis-EC AriE from Amycolatopsis arida, which catalyzes geranylgeranyl diphosphate to form cis-6/10-fused bicyclic benditerpe-2,6,15-triene (1) in aridacin biosynthesis. Through surface entropy reduction, we obtained the 1.87 A resolution structure of AriED128A. Structure-guided mutagenesis identified W73 as a key regulatory hotspot controlling product diversity, with its variants generating diverse diterpene products. Y203A converted AriE into a highly specific (-)-(R)-cembrene A synthase. Our findings provide the first structural insights into this type of terpene synthase and demonstrate the feasibility of engineering EC variants for selective product formation.


Authors: Li, F.R., Yang, Q., He, J.Y., Sun, X.R., Pan, X.M., Xu, H.M., Rudolf, J.D., Dong, L.B.
Crystal Structure and Catalytic Mechanism of the cis-Eunicellane Cyclase AriE.,Li FR, Yang Q, He J, Sun X, Pan X, Xu HM, Rudolf JD, Dong LB Chemistry. 2025 Apr 23:e202500012. doi: 10.1002/chem.202500012. PMID:40265331<ref>PMID:40265331</ref>


Description: Terpene cyclase AriE mutant-D128A
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Li, F.R]]
<div class="pdbe-citations 9lpb" style="background-color:#fffaf0;"></div>
[[Category: He, J.Y]]
== References ==
[[Category: Dong, L.B]]
<references/>
[[Category: Xu, H.M]]
__TOC__
[[Category: Rudolf, J.D]]
</StructureSection>
[[Category: Pan, X.M]]
[[Category: Amycolatopsis arida]]
[[Category: Sun, X.R]]
[[Category: Large Structures]]
[[Category: Yang, Q]]
[[Category: Dong LB]]
[[Category: He JY]]
[[Category: Li FR]]
[[Category: Pan XM]]
[[Category: Rudolf JD]]
[[Category: Sun XR]]
[[Category: Xu HM]]
[[Category: Yang Q]]
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