8bbz

Crystal Structure of SilF (apo form)

spinqualitylabelsall models 8bbz, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8bbz is a 3 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.2Å Ligands: ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A482M8M0_ECOLX Publication Abstract from PubMed The periplasmic chaperone SilF has been identified as part of an Ag(I) detoxification system in Gram-negative bacteria. Sil proteins also bind Cu(I) but with reported weaker affinity, therefore leading to the designation of a specific detoxification system for Ag(I). Using isothermal titration calorimetry, we show that binding of both ions is not only tighter than previously thought but of very similar affinities. We investigated the structural origins of ion binding using molecular dynamics and QM/MM simulations underpinned by structural and biophysical experiments. The results of this analysis showed that the binding site adapts to accommodate either ion, with key interactions with the solvent in the case of Cu(I). The implications of this are that Gram-negative bacteria do not appear to have evolved a specific Ag(I) efflux system but take advantage of the existing Cu(I) detoxification system. Therefore, there are consequences for how we define a particular metal resistance mechanism and understand its evolution in the environment. The adaptability of the ion-binding site by the Ag(I)/Cu(I) periplasmic chaperone SilF.,Lithgo RM, Hanzevacki M, Harris G, Kamps JJAG, Holden E, Gianga TM, Benesch JLP, Jager CM, Croft AK, Hussain R, Hobman JL, Orville AM, Quigley A, Carr SB, Scott DJ J Biol Chem. 2023 Nov;299(11):105331. doi: 10.1016/j.jbc.2023.105331. Epub 2023 , Oct 14. PMID:37820867 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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