1vro: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1vro.gif|left|200px]] | [[Image:1vro.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1vro", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_1vro| PDB=1vro | SCENE= }} | |||
}} | |||
'''Selenium-Assisted Nucleic Acid Crystallography: Use of Phosphoroselenoates for MAD Phasing of a DNA Structure''' | '''Selenium-Assisted Nucleic Acid Crystallography: Use of Phosphoroselenoates for MAD Phasing of a DNA Structure''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1n6s 1n6s]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VRO OCA]. | |||
==Reference== | ==Reference== | ||
Selenium-assisted nucleic acid crystallography: use of phosphoroselenoates for MAD phasing of a DNA structure., Wilds CJ, Pattanayek R, Pan C, Wawrzak Z, Egli M, J Am Chem Soc. 2002 Dec 18;124(50):14910-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12475332 12475332] | Selenium-assisted nucleic acid crystallography: use of phosphoroselenoates for MAD phasing of a DNA structure., Wilds CJ, Pattanayek R, Pan C, Wawrzak Z, Egli M, J Am Chem Soc. 2002 Dec 18;124(50):14910-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12475332 12475332] | ||
[[Category: Egli, M.]] | [[Category: Egli, M.]] | ||
[[Category: Pan, C.]] | [[Category: Pan, C.]] | ||
| Line 29: | Line 25: | ||
[[Category: Wawrzak, Z.]] | [[Category: Wawrzak, Z.]] | ||
[[Category: Wilds, C J.]] | [[Category: Wilds, C J.]] | ||
[[Category: | [[Category: Covalent modification of oligonucleotide]] | ||
[[Category: | [[Category: Left-handed z-dna]] | ||
[[Category: | [[Category: Oligonucleotide analogue]] | ||
[[Category: Phasing strategy]] | |||
[[Category: | [[Category: Phosphoroselenoate]] | ||
[[Category: | [[Category: Synchrotron]] | ||
[[Category: | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:50:27 2008'' | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 09:50, 3 May 2008
Selenium-Assisted Nucleic Acid Crystallography: Use of Phosphoroselenoates for MAD Phasing of a DNA Structure
Overview
The combination of synchrotron radiation and a variety of atoms or ions (either covalently attached to the biomolecule prior to crystallization or soaked into crystals) that serve as anomalous scatterers constitutes a powerful tool in the X-ray crystallographer's repertoire of structure determination techniques. Phosphoroselenoates in which one of the nonbridging phosphate oxygens in the backbone is replaced by selenium offer a simplified means for introducing an anomalous scatterer into oligonucleotides by conventional solid-phase synthesis. Unlike other methods that are used to derivatize DNA or RNA by covalent attachment of a heavy atom (i.e., bromine at the C5 position of pyrimidines), tedious synthesis of specialized nucleosides is not required. Introduction of selenium is readily accomplished in solid-phase oligonucleotide synthesis by replacing the standard oxidation agent with a solution of potassium selenocyanide. This results in a diastereomeric mixture of phosphoroselenoates that can be separated by strong anion-exchange HPLC. As a test case, all 10 DNA hexamers of the sequence CGCGCG containing a single phosphoroselenoate linkage (PSe) were prepared. Crystals were grown for a subset of them, and the structure of [d(C(PSe)GCGCG)](2) was determined by the multiwavelength anomalous dispersion technique and refined to 1.1 A resolution.
About this Structure
This structure supersedes the now removed PDB entry 1n6s. Full crystallographic information is available from OCA.
Reference
Selenium-assisted nucleic acid crystallography: use of phosphoroselenoates for MAD phasing of a DNA structure., Wilds CJ, Pattanayek R, Pan C, Wawrzak Z, Egli M, J Am Chem Soc. 2002 Dec 18;124(50):14910-6. PMID:12475332 Page seeded by OCA on Sat May 3 12:50:27 2008
