6wu0: Difference between revisions
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<StructureSection load='6wu0' size='340' side='right'caption='[[6wu0]], [[Resolution|resolution]] 3.59Å' scene=''> | <StructureSection load='6wu0' size='340' side='right'caption='[[6wu0]], [[Resolution|resolution]] 3.59Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WU0 FirstGlance]. <br> | ||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.59Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.59Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wu0 OCA], [https://pdbe.org/6wu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wu0 RCSB], [https://www.ebi.ac.uk/pdbsum/6wu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wu0 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wu0 OCA], [https://pdbe.org/6wu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wu0 RCSB], [https://www.ebi.ac.uk/pdbsum/6wu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wu0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | |||
Single-particle cryo-electron microscopy (cryo-EM) has become a powerful technique in the field of structural biology. However, the inability to reliably produce pure, homogeneous membrane protein samples hampers the progress of their structural determination. Here, we develop a bottom-up iterative method, Build and Retrieve (BaR), that enables the identification and determination of cryo-EM structures of a variety of inner and outer membrane proteins, including membrane protein complexes of different sizes and dimensions, from a heterogeneous, impure protein sample. We also use the BaR methodology to elucidate structural information from Escherichia coli K12 crude membrane and raw lysate. The findings demonstrate that it is possible to solve high-resolution structures of a number of relatively small (<100 kDa) and less abundant (<10%) unidentified membrane proteins within a single, heterogeneous sample. Importantly, these results highlight the potential of cryo-EM for systems structural proteomics. | |||
A 'Build and Retrieve' methodology to simultaneously solve cryo-EM structures of membrane proteins.,Su CC, Lyu M, Morgan CE, Bolla JR, Robinson CV, Yu EW Nat Methods. 2021 Jan;18(1):69-75. doi: 10.1038/s41592-020-01021-2. Epub 2021 Jan, 6. PMID:33408407<ref>PMID:33408407</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6wu0" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Lyu M]] | [[Category: Lyu M]] | ||
[[Category: Yu EW]] | [[Category: Yu EW]] | ||
Latest revision as of 05:45, 28 May 2025
CryoEM structure of Burkholderia pseudomallei hopanoid biosynthesis-associated RND transporter HpnN
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