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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7x7a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Scalindua_brodae Candidatus Scalindua brodae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X7A FirstGlance]. <br>
<table><tr><td colspan='2'>[[7x7a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Scalindua_brodae Candidatus Scalindua brodae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X7A FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x7a OCA], [https://pdbe.org/7x7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x7a RCSB], [https://www.ebi.ac.uk/pdbsum/7x7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x7a ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x7a OCA], [https://pdbe.org/7x7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x7a RCSB], [https://www.ebi.ac.uk/pdbsum/7x7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x7a ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/A0A0B0EGF3_9BACT A0A0B0EGF3_9BACT]  
[https://www.uniprot.org/uniprot/A0A0B0EGF3_9BACT A0A0B0EGF3_9BACT]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The type III-E CRISPR-Cas system uses a single multidomain effector called Cas7-11 (also named gRAMP) to cleave RNA and associate with a caspase-like protease Csx29, showing promising potential for RNA-targeting applications. The structural and molecular mechanisms of the type III-E CRISPR-Cas system remain poorly understood. Here we report four cryo-electron microscopy structures of Cas7-11 at different functional states. Cas7-11 has four Cas7-like domains, which assemble into a helical filament to accommodate CRISPR RNA (crRNA), and a Cas11-like domain facilitating crRNA-target RNA duplex formation. The Cas7.1 domain is critical for crRNA maturation, whereas Cas7.2 and Cas7.3 are responsible for target RNA cleavage. Target RNA binding induces the structural arrangements of Csx29, potentially exposing the catalytic site of Csx29. These results delineate the molecular mechanisms underlying pre-crRNA processing, target RNA recognition and cleavage for Cas7-11, and provide a structural framework to understand the role of Csx29 in type III-E CRISPR system.
Structure and function of a bacterial type III-E CRISPR-Cas7-11 complex.,Yu G, Wang X, Zhang Y, An Q, Wen Y, Li X, Yin H, Deng Z, Zhang H Nat Microbiol. 2022 Oct 27. pii: 10.1038/s41564-022-01256-z. doi:, 10.1038/s41564-022-01256-z. PMID:36302881<ref>PMID:36302881</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7x7a" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>

Latest revision as of 06:43, 25 June 2025

Cryo-EM structure of SbCas7-11 in complex with crRNA and target RNA

7x7a, resolution 3.20Å

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