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<StructureSection load='7whp' size='340' side='right'caption='[[7whp]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
<StructureSection load='7whp' size='340' side='right'caption='[[7whp]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7whp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WHP FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WHP FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7whp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7whp OCA], [https://pdbe.org/7whp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7whp RCSB], [https://www.ebi.ac.uk/pdbsum/7whp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7whp ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7whp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7whp OCA], [https://pdbe.org/7whp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7whp RCSB], [https://www.ebi.ac.uk/pdbsum/7whp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7whp ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Many viruses utilize trimeric spikes to gain entry into host cells. However, without in situ structures of these trimeric spikes, a full understanding of this dynamic and essential process of viral infections is not possible. Here we present four in situ and one isolated cryoEM structures of the trimeric spike of the cytoplasmic polyhedrosis virus, a member of the non-enveloped Reoviridae family and a virus historically used as a model in the discoveries of RNA transcription and capping. These structures adopt two drastically different conformations, closed spike and opened spike, which respectively represent the penetration-inactive and penetration-active states. Each spike monomer has four domains: N-terminal, body, claw, and C-terminal. From closed to opened state, the RGD motif-containing C-terminal domain is freed to bind integrins, and the claw domain rotates to expose and project its membrane insertion loops into the cellular membrane. Comparison between turret vertices before and after detachment of the trimeric spike shows that the trimeric spike anchors its N-terminal domain in the iris of the pentameric RNA-capping turret. Sensing of cytosolic S-adenosylmethionine (SAM) and adenosine triphosphate (ATP) by the turret triggers a cascade of events: opening of the iris, detachment of the spike, and initiation of endogenous transcription.
Multiple conformations of trimeric spikes visualized on a non-enveloped virus.,Zhang Y, Cui Y, Sun J, Zhou ZH Nat Commun. 2022 Jan 27;13(1):550. doi: 10.1038/s41467-022-28114-0. PMID:35087065<ref>PMID:35087065</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7whp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bombyx mori cypovirus 1]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Cui, Y]]
[[Category: Cui Y]]
[[Category: Sun, J]]
[[Category: Sun J]]
[[Category: Zhang, Y]]
[[Category: Zhang Y]]
[[Category: Zhou, Z H]]
[[Category: Zhou ZH]]
[[Category: Capping enzyme]]
[[Category: Viral protein]]

Latest revision as of 06:21, 3 July 2025

Pentameric turret of Bombyx mori cytoplasmic polyhedrosis virus after spike detaches.

7whp, resolution 3.70Å

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