8q75: Difference between revisions
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==Copper-transporting ATPase HMA4 in E2P state with AlF== | |||
<StructureSection load='8q75' size='340' side='right'caption='[[8q75]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8q75]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8Q75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8Q75 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8q75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8q75 OCA], [https://pdbe.org/8q75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8q75 RCSB], [https://www.ebi.ac.uk/pdbsum/8q75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8q75 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Copper transporting P-type (P(1B-1)-) ATPases are essential for cellular homeostasis. Nonetheless, the E1-E1P-E2P-E2 states mechanism of P(1B-1)-ATPases remains poorly understood. In particular, the role of the intrinsic metal binding domains (MBDs) is enigmatic. Here, four cryo-EM structures and molecular dynamics simulations of a P(1B-1)-ATPase are combined to reveal that in many eukaryotes the MBD immediately prior to the ATPase core, MBD(-1), serves a structural role, remodeling the ion-uptake region. In contrast, the MBD prior to MBD(-1), MBD(-2), likely assists in copper delivery to the ATPase core. Invariant Tyr, Asn and Ser residues in the transmembrane domain assist in positioning sulfur-providing copper-binding amino acids, allowing for copper uptake, binding and release. As such, our findings unify previously conflicting data on the transport and regulation of P(1B-1)-ATPases. The results are critical for a fundamental understanding of cellular copper homeostasis and for comprehension of the molecular bases of P(1B-1)-disorders and ongoing clinical trials. | |||
Diverse roles of the metal binding domains and transport mechanism of copper transporting P-type ATPases.,Guo Z, Oradd F, Bagenholm V, Gronberg C, Ma JF, Ott P, Wang Y, Andersson M, Pedersen PA, Wang K, Gourdon P Nat Commun. 2024 Mar 27;15(1):2690. doi: 10.1038/s41467-024-47001-4. PMID:38538615<ref>PMID:38538615</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8q75" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Oryza sativa Japonica Group]] | |||
[[Category: Gourdon P]] | |||
[[Category: Guo Z]] | |||
[[Category: Wang K]] | |||
Latest revision as of 07:03, 3 July 2025
Copper-transporting ATPase HMA4 in E2P state with AlF
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