9luo: Difference between revisions

Latest revision as of 16:24, 9 July 2025

Cryo-EM structure of Arabidopsis thaliana RGA in complex with GID1A, SLY1, and ASK2 (focused map)

Structural highlights

9luo is a 3 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.07Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RGA_ARATH Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Positively regulates XERICO expression in seeds. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. Compared to other DELLA proteins, it is the most sensitive to GA application. No effect of the BOI proteins on its stability. Its activity is probably regulated by other phytohormones such as auxin and ethylene, attenuation of auxin transport delaying its GA-induced degradation. Involved in the regulation of seed dormancy and germination, including glucose-induced delay of seed germination (PubMed:24989044).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8] ^[9] ^[10] ^[11]

References

↑ King KE, Moritz T, Harberd NP. Gibberellins are not required for normal stem growth in Arabidopsis thaliana in the absence of GAI and RGA. Genetics. 2001 Oct;159(2):767-76. PMID:11606551
↑ Dill A, Sun T. Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana. Genetics. 2001 Oct;159(2):777-85. PMID:11606552
↑ Fu X, Harberd NP. Auxin promotes Arabidopsis root growth by modulating gibberellin response. Nature. 2003 Feb 13;421(6924):740-3. PMID:12610625 doi:10.1038/nature01387
↑ Achard P, Vriezen WH, Van Der Straeten D, Harberd NP. Ethylene regulates arabidopsis development via the modulation of DELLA protein growth repressor function. Plant Cell. 2003 Dec;15(12):2816-25. PMID:14615596 doi:10.1105/tpc.015685
↑ Cheng H, Qin L, Lee S, Fu X, Richards DE, Cao D, Luo D, Harberd NP, Peng J. Gibberellin regulates Arabidopsis floral development via suppression of DELLA protein function. Development. 2004 Mar;131(5):1055-64. PMID:14973286 doi:https://dx.doi.org/10.1242/dev.00992
↑ Yu H, Ito T, Zhao Y, Peng J, Kumar P, Meyerowitz EM. Floral homeotic genes are targets of gibberellin signaling in flower development. Proc Natl Acad Sci U S A. 2004 May 18;101(20):7827-32. Epub 2004 May 5. PMID:15128937 doi:https://dx.doi.org/10.1073/pnas.0402377101
↑ Cao D, Hussain A, Cheng H, Peng J. Loss of function of four DELLA genes leads to light- and gibberellin-independent seed germination in Arabidopsis. Planta. 2005 Dec;223(1):105-13. Epub 2005 Jul 21. PMID:16034591 doi:https://dx.doi.org/10.1007/s00425-005-0057-3
↑ Zentella R, Zhang ZL, Park M, Thomas SG, Endo A, Murase K, Fleet CM, Jikumaru Y, Nambara E, Kamiya Y, Sun TP. Global analysis of della direct targets in early gibberellin signaling in Arabidopsis. Plant Cell. 2007 Oct;19(10):3037-57. Epub 2007 Oct 12. PMID:17933900 doi:https://dx.doi.org/10.1105/tpc.107.054999
↑ Gallego-Bartolomé J, Minguet EG, Marín JA, Prat S, Blázquez MA, Alabadí D. Transcriptional diversification and functional conservation between DELLA proteins in Arabidopsis. Mol Biol Evol. 2010 Jun;27(6):1247-56. PMID:20093430 doi:10.1093/molbev/msq012
↑ Rombolá-Caldentey B, Rueda-Romero P, Iglesias-Fernández R, Carbonero P, Oñate-Sánchez L. Arabidopsis DELLA and two HD-ZIP transcription factors regulate GA signaling in the epidermis through the L1 box cis-element. Plant Cell. 2014 Jul;26(7):2905-19. PMID:24989044 doi:10.1105/tpc.114.127647
↑ Silverstone AL, Ciampaglio CN, Sun T. The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway. Plant Cell. 1998 Feb;10(2):155-69. PMID:9490740 doi:10.1105/tpc.10.2.155

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OCA

[1] King KE, Moritz T, Harberd NP. Gibberellins are not required for normal stem growth in Arabidopsis thaliana in the absence of GAI and RGA. Genetics. 2001 Oct;159(2):767-76. PMID:11606551

[2] Dill A, Sun T. Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana. Genetics. 2001 Oct;159(2):777-85. PMID:11606552

[3] Fu X, Harberd NP. Auxin promotes Arabidopsis root growth by modulating gibberellin response. Nature. 2003 Feb 13;421(6924):740-3. PMID:12610625 doi:10.1038/nature01387

[4] Achard P, Vriezen WH, Van Der Straeten D, Harberd NP. Ethylene regulates arabidopsis development via the modulation of DELLA protein growth repressor function. Plant Cell. 2003 Dec;15(12):2816-25. PMID:14615596 doi:10.1105/tpc.015685

[5] Cheng H, Qin L, Lee S, Fu X, Richards DE, Cao D, Luo D, Harberd NP, Peng J. Gibberellin regulates Arabidopsis floral development via suppression of DELLA protein function. Development. 2004 Mar;131(5):1055-64. PMID:14973286 doi:https://dx.doi.org/10.1242/dev.00992

[6] Yu H, Ito T, Zhao Y, Peng J, Kumar P, Meyerowitz EM. Floral homeotic genes are targets of gibberellin signaling in flower development. Proc Natl Acad Sci U S A. 2004 May 18;101(20):7827-32. Epub 2004 May 5. PMID:15128937 doi:https://dx.doi.org/10.1073/pnas.0402377101

[7] Cao D, Hussain A, Cheng H, Peng J. Loss of function of four DELLA genes leads to light- and gibberellin-independent seed germination in Arabidopsis. Planta. 2005 Dec;223(1):105-13. Epub 2005 Jul 21. PMID:16034591 doi:https://dx.doi.org/10.1007/s00425-005-0057-3

[8] Zentella R, Zhang ZL, Park M, Thomas SG, Endo A, Murase K, Fleet CM, Jikumaru Y, Nambara E, Kamiya Y, Sun TP. Global analysis of della direct targets in early gibberellin signaling in Arabidopsis. Plant Cell. 2007 Oct;19(10):3037-57. Epub 2007 Oct 12. PMID:17933900 doi:https://dx.doi.org/10.1105/tpc.107.054999

[9] Gallego-Bartolomé J, Minguet EG, Marín JA, Prat S, Blázquez MA, Alabadí D. Transcriptional diversification and functional conservation between DELLA proteins in Arabidopsis. Mol Biol Evol. 2010 Jun;27(6):1247-56. PMID:20093430 doi:10.1093/molbev/msq012

[10] Rombolá-Caldentey B, Rueda-Romero P, Iglesias-Fernández R, Carbonero P, Oñate-Sánchez L. Arabidopsis DELLA and two HD-ZIP transcription factors regulate GA signaling in the epidermis through the L1 box cis-element. Plant Cell. 2014 Jul;26(7):2905-19. PMID:24989044 doi:10.1105/tpc.114.127647

[11] Silverstone AL, Ciampaglio CN, Sun T. The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway. Plant Cell. 1998 Feb;10(2):155-69. PMID:9490740 doi:10.1105/tpc.10.2.155

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9luo is ON HOLD  until Paper Publication
+==Cryo-EM structure of Arabidopsis thaliana RGA in complex with GID1A, SLY1, and ASK2 (focused map)==
+<StructureSection load='9luo' size='340' side='right'caption='[[9luo]], [[Resolution|resolution]] 3.07&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9luo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LUO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LUO FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.07&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9luo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9luo OCA], [https://pdbe.org/9luo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9luo RCSB], [https://www.ebi.ac.uk/pdbsum/9luo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9luo ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RGA_ARATH RGA_ARATH] Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Positively regulates XERICO expression in seeds. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. Compared to other DELLA proteins, it is the most sensitive to GA application. No effect of the BOI proteins on its stability. Its activity is probably regulated by other phytohormones such as auxin and ethylene, attenuation of auxin transport delaying its GA-induced degradation. Involved in the regulation of seed dormancy and germination, including glucose-induced delay of seed germination (PubMed:24989044).<ref>PMID:11606551</ref> <ref>PMID:11606552</ref> <ref>PMID:12610625</ref> <ref>PMID:14615596</ref> <ref>PMID:14973286</ref> <ref>PMID:15128937</ref> <ref>PMID:16034591</ref> <ref>PMID:17933900</ref> <ref>PMID:20093430</ref> <ref>PMID:24989044</ref> <ref>PMID:9490740</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Arabidopsis thaliana]]
+[[Category: Large Structures]]
+[[Category: Islam S]]
+[[Category: Kim DY]]
+[[Category: Kwon E]]
+[[Category: Park K]]

9luo: Difference between revisions

Latest revision as of 16:24, 9 July 2025

Cryo-EM structure of Arabidopsis thaliana RGA in complex with GID1A, SLY1, and ASK2 (focused map)

Structural highlights

Function

References

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