8h95: Difference between revisions

Latest revision as of 06:09, 23 July 2025

Structure of mouse SCMC bound with full-length FILIA

Structural highlights

8h95 is a 3 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.38Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NALP5_MOUSE Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development (PubMed:11062459, PubMed:18804437, PubMed:25208553, PubMed:37922900). The SCMC complex is a structural constituent of cytoplasmic lattices, which consist in fibrous structures found in the cytoplasm of oocytes and preimplantation embryos (PubMed:37922900). They are required to store maternal proteins critical for embryonic development, such as proteins that control epigenetic reprogramming of the preimplantation embryo, and prevent their degradation or activation (PubMed:37922900). Required for the localization of cortical granules to the cortex of oocytes, via association with the cortical actin scaffold (PubMed:31118423). Required for cortical actin clearance prior to oocyte exocytosis and prevention of polyspermy (PubMed:31118423). Involved in regulating post-fertilization Ca(2+) release and endoplasmic reticulum storage (ER) storage via regulation of cellular localization (PubMed:24374158). May be involved in the localization of mitochondria to the cytoplasm and perinuclear region in oocytes and early stage embryos, independent of its role in CPL formation (PubMed:22357545).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

References

↑ Tong ZB, Gold L, Pfeifer KE, Dorward H, Lee E, Bondy CA, Dean J, Nelson LM. Mater, a maternal effect gene required for early embryonic development in mice. Nat Genet. 2000 Nov;26(3):267-8. PMID:11062459 doi:10.1038/81547
↑ Li L, Baibakov B, Dean J. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev Cell. 2008 Sep;15(3):416-425. PMID:18804437 doi:10.1016/j.devcel.2008.07.010
↑ Fernandes R, Tsuda C, Perumalsamy AL, Naranian T, Chong J, Acton BM, Tong ZB, Nelson LM, Jurisicova A. NLRP5 mediates mitochondrial function in mouse oocytes and embryos. Biol Reprod. 2012 May 3;86(5):138, 1-10. PMID:22357545 doi:10.1095/biolreprod.111.093583
↑ Kim B, Zhang X, Kan R, Cohen R, Mukai C, Travis AJ, Coonrod SA. The role of MATER in endoplasmic reticulum distribution and calcium homeostasis in mouse oocytes. Dev Biol. 2014 Feb 15;386(2):331-9. PMID:24374158 doi:10.1016/j.ydbio.2013.12.025
↑ Yu XJ, Yi Z, Gao Z, Qin D, Zhai Y, Chen X, Ou-Yang Y, Wang ZB, Zheng P, Zhu MS, Wang H, Sun QY, Dean J, Li L. The subcortical maternal complex controls symmetric division of mouse zygotes by regulating F-actin dynamics. Nat Commun. 2014 Sep 11;5:4887. PMID:25208553 doi:10.1038/ncomms5887
↑ Vogt EJ, Tokuhiro K, Guo M, Dale R, Yang G, Shin SW, Movilla MJ, Shroff H, Dean J. Anchoring cortical granules in the cortex ensures trafficking to the plasma membrane for post-fertilization exocytosis. Nat Commun. 2019 May 22;10(1):2271. PMID:31118423 doi:10.1038/s41467-019-10171-7
↑ Jentoft IMA, Bäuerlein FJB, Welp LM, Cooper BH, Petrovic A, So C, Penir SM, Politi AZ, Horokhovskyi Y, Takala I, Eckel H, Moltrecht R, Lénárt P, Cavazza T, Liepe J, Brose N, Urlaub H, Fernández-Busnadiego R, Schuh M. Mammalian oocytes store proteins for the early embryo on cytoplasmic lattices. Cell. 2023 Nov 22;186(24):5308-5327.e25. PMID:37922900 doi:10.1016/j.cell.2023.10.003

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OCA

[1] Tong ZB, Gold L, Pfeifer KE, Dorward H, Lee E, Bondy CA, Dean J, Nelson LM. Mater, a maternal effect gene required for early embryonic development in mice. Nat Genet. 2000 Nov;26(3):267-8. PMID:11062459 doi:10.1038/81547

[2] Li L, Baibakov B, Dean J. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev Cell. 2008 Sep;15(3):416-425. PMID:18804437 doi:10.1016/j.devcel.2008.07.010

[3] Fernandes R, Tsuda C, Perumalsamy AL, Naranian T, Chong J, Acton BM, Tong ZB, Nelson LM, Jurisicova A. NLRP5 mediates mitochondrial function in mouse oocytes and embryos. Biol Reprod. 2012 May 3;86(5):138, 1-10. PMID:22357545 doi:10.1095/biolreprod.111.093583

[4] Kim B, Zhang X, Kan R, Cohen R, Mukai C, Travis AJ, Coonrod SA. The role of MATER in endoplasmic reticulum distribution and calcium homeostasis in mouse oocytes. Dev Biol. 2014 Feb 15;386(2):331-9. PMID:24374158 doi:10.1016/j.ydbio.2013.12.025

[5] Yu XJ, Yi Z, Gao Z, Qin D, Zhai Y, Chen X, Ou-Yang Y, Wang ZB, Zheng P, Zhu MS, Wang H, Sun QY, Dean J, Li L. The subcortical maternal complex controls symmetric division of mouse zygotes by regulating F-actin dynamics. Nat Commun. 2014 Sep 11;5:4887. PMID:25208553 doi:10.1038/ncomms5887

[6] Vogt EJ, Tokuhiro K, Guo M, Dale R, Yang G, Shin SW, Movilla MJ, Shroff H, Dean J. Anchoring cortical granules in the cortex ensures trafficking to the plasma membrane for post-fertilization exocytosis. Nat Commun. 2019 May 22;10(1):2271. PMID:31118423 doi:10.1038/s41467-019-10171-7

[7] Jentoft IMA, Bäuerlein FJB, Welp LM, Cooper BH, Petrovic A, So C, Penir SM, Politi AZ, Horokhovskyi Y, Takala I, Eckel H, Moltrecht R, Lénárt P, Cavazza T, Liepe J, Brose N, Urlaub H, Fernández-Busnadiego R, Schuh M. Mammalian oocytes store proteins for the early embryo on cytoplasmic lattices. Cell. 2023 Nov 22;186(24):5308-5327.e25. PMID:37922900 doi:10.1016/j.cell.2023.10.003

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 </table>
 == Function ==
-[https://www.uniprot.org/uniprot/NALP5_MOUSE NALP5_MOUSE] As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:11062459, PubMed:18804437, PubMed:25208553). Required for the formation of F-actin cytoplasmic lattices (CPL) in oocytes, which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning (PubMed:25208553). Required for the localization of cortical granules to the cortex of oocytes, via association with the cortical actin scaffold (PubMed:31118423). Required for cortical actin clearance prior to oocyte exocytosis and prevention of polyspermy (PubMed:31118423). Involved in regulating post-fertilization Ca(2+) release and endoplasmic reticulum storage (ER) storage via regulation of cellular localization (PubMed:24374158). May be involved in the localization of mitochondria to the cytoplasm and perinuclear region in oocytes and early stage embryos, independent of its role in CPL formation (PubMed:22357545).<ref>PMID:11062459</ref> <ref>PMID:18804437</ref> <ref>PMID:22357545</ref> <ref>PMID:24374158</ref> <ref>PMID:25208553</ref> <ref>PMID:31118423</ref>
+[https://www.uniprot.org/uniprot/NALP5_MOUSE NALP5_MOUSE] Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development (PubMed:11062459, PubMed:18804437, PubMed:25208553, PubMed:37922900). The SCMC complex is a structural constituent of cytoplasmic lattices, which consist in fibrous structures found in the cytoplasm of oocytes and preimplantation embryos (PubMed:37922900). They are required to store maternal proteins critical for embryonic development, such as proteins that control epigenetic reprogramming of the preimplantation embryo, and prevent their degradation or activation (PubMed:37922900). Required for the localization of cortical granules to the cortex of oocytes, via association with the cortical actin scaffold (PubMed:31118423). Required for cortical actin clearance prior to oocyte exocytosis and prevention of polyspermy (PubMed:31118423). Involved in regulating post-fertilization Ca(2+) release and endoplasmic reticulum storage (ER) storage via regulation of cellular localization (PubMed:24374158). May be involved in the localization of mitochondria to the cytoplasm and perinuclear region in oocytes and early stage embryos, independent of its role in CPL formation (PubMed:22357545).<ref>PMID:11062459</ref> <ref>PMID:18804437</ref> <ref>PMID:22357545</ref> <ref>PMID:24374158</ref> <ref>PMID:25208553</ref> <ref>PMID:31118423</ref> <ref>PMID:37922900</ref>
 == References ==
 <references/>

8h95: Difference between revisions

Latest revision as of 06:09, 23 July 2025

Structure of mouse SCMC bound with full-length FILIA

Structural highlights

Function

References

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