1wbp: Difference between revisions
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'''SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE''' | '''SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE''' | ||
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[[Category: Nolen, B.]] | [[Category: Nolen, B.]] | ||
[[Category: Velazquez-Dones, A.]] | [[Category: Velazquez-Dones, A.]] | ||
[[Category: | [[Category: Alternative splicing]] | ||
[[Category: | [[Category: Atp-binding]] | ||
[[Category: | [[Category: Chromosome partition]] | ||
[[Category: | [[Category: Differentiation]] | ||
[[Category: | [[Category: Kinase]] | ||
[[Category: | [[Category: Mrna processing]] | ||
[[Category: | [[Category: Mrna splicing]] | ||
[[Category: | [[Category: Nuclear protein]] | ||
[[Category: | [[Category: Nucleotide-binding]] | ||
[[Category: | [[Category: Phosphorylation]] | ||
[[Category: | [[Category: Serine/threonine-protein kinase]] | ||
[[Category: | [[Category: Srpk]] | ||
[[Category: | [[Category: Transferase]] | ||
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Revision as of 10:25, 3 May 2008
SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE
Overview
The arginine-serine (RS)-rich domain of the SR protein ASF/SF2 is phosphorylated by SR protein kinases (SRPKs) and Clk/Sty kinases. However, the mode of phosphorylation by these kinases and their coordination in the biological regulation of ASF/SF2 is unknown. Here, we report the crystal structure of an active fragment of human SRPK1 bound to a peptide derived from an SR protein. This structure led us to identify a docking motif in ASF/SF2. We find that this docking motif restricts phosphorylation of ASF/SF2 by SRPK1 to the N-terminal part of the RS domain - a property essential for its assembly into nuclear speckles. We further show that Clk/Sty causes release of ASF/SF2 from speckles by phosphorylating the C-terminal part of its RS domain. These results suggest that the docking motif of ASF/SF2 is a key regulatory element for sequential phosphorylation by SRPK1 and Clk/Sty and, thus, is essential for its subcellular localization.
About this Structure
1WBP is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Interplay between SRPK and Clk/Sty kinases in phosphorylation of the splicing factor ASF/SF2 is regulated by a docking motif in ASF/SF2., Ngo JC, Chakrabarti S, Ding JH, Velazquez-Dones A, Nolen B, Aubol BE, Adams JA, Fu XD, Ghosh G, Mol Cell. 2005 Oct 7;20(1):77-89. PMID:16209947 Page seeded by OCA on Sat May 3 13:25:52 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Homo sapiens
- Non-specific serine/threonine protein kinase
- Protein complex
- Adams, J A.
- Aubol, B E.
- Chakrabarti, S.
- Ding, J H.
- Fu, X D.
- Ghosh, G.
- Ngo, J C.
- Nolen, B.
- Velazquez-Dones, A.
- Alternative splicing
- Atp-binding
- Chromosome partition
- Differentiation
- Kinase
- Mrna processing
- Mrna splicing
- Nuclear protein
- Nucleotide-binding
- Phosphorylation
- Serine/threonine-protein kinase
- Srpk
- Transferase
