9j25: Difference between revisions

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'''Unreleased structure'''


The entry 9j25 is ON HOLD  until Paper Publication
==Structural basis of the bifunctionality of M. salinexigens ZYF650T glucosylglycerol phosphorylase in glucosylglycerol catabolism==
<StructureSection load='9j25' size='340' side='right'caption='[[9j25]], [[Resolution|resolution]] 2.74&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9j25]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinobacter_salinexigens Marinobacter salinexigens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9J25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9J25 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.74&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9j25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9j25 OCA], [https://pdbe.org/9j25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9j25 RCSB], [https://www.ebi.ac.uk/pdbsum/9j25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9j25 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A5B0VBK8_9GAMM A0A5B0VBK8_9GAMM]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
2-O-alpha-Glucosylglycerol (GG) is a natural heteroside synthesized by many cyanobacteria and a few heterotrophic bacteria under salt stress conditions. Bacteria produce GG in response to stimuli and degrade it once the stimulus diminishes. Heterotrophic bacteria utilize GG phosphorylase (GGP), a member of the GH13_18 family, via a two-step process consisting of phosphorolysis and hydrolysis for GG catabolism. However, the precise mechanism by which GGP degrades GG remains elusive. We determined the 3D structure of a recently identified GGP (MsGGP) of the deep-sea bacterium Marinobacter salinexigens ZYF650(T), in complex with glucose and glycerol, alpha-d-glucose-1-phosphate (alphaGlc1-P), and orthophosphate (inorganic phosphate) at resolutions of 2.5, 2.7, and 2.7 A, respectively. Notably, the first alphaGlc1-P complex structure in the GH13_18 family, the complex of MsGGP and alphaGlc1-P, validates that GGP catalyzes GG decomposition through consecutive phosphorolysis and hydrolysis. In addition, the structure reveals the mechanism of high stereoselectivity on alphaGlc1-P. Glu231 and Asp190 were identified as the catalytic residues. Interestingly, these structures closely resemble each other, indicating minimal conformational changes upon binding end-product glucose and glycerol, or the intermediate alphaGlc1-P. The structures also indicate that the substrates may follow a specific trajectory and a precise order toward the active center in close proximity and in a geometrically favorable orientation for catalysis in a double displacement mechanism.


Authors: Lu, D., Ma, H.L.
Structural basis of the bifunctionality of Marinobacter salinexigens ZYF650(T) glucosylglycerol phosphorylase in glucosylglycerol catabolism.,Lu D, Zhang K, Cheng C, Wu D, Yin L, Luo Q, Shi M, Ma H, Lu X J Biol Chem. 2025 Feb;301(2):108127. doi: 10.1016/j.jbc.2024.108127. Epub 2024 , Dec 25. PMID:39725037<ref>PMID:39725037</ref>


Description: Structural basis of the bifunctionality of M. salinexigens ZYF650T glucosylglycerol phosphorylase in glucosylglycerol catabolism
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Ma, H.L]]
<div class="pdbe-citations 9j25" style="background-color:#fffaf0;"></div>
[[Category: Lu, D]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Marinobacter salinexigens]]
[[Category: Lu D]]
[[Category: Ma HL]]
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