9sh8: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
m Protected "9sh8" [edit=sysop:move=sysop]
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
Line 1: Line 1:
'''Unreleased structure'''


The entry 9sh8 is ON HOLD
==Thermus thermophilus asparaginyl-tRNA synthetase with bound sulphamoyl-analogue of asparaginyl-adenylate (AsnAMS)==
<StructureSection load='9sh8' size='340' side='right'caption='[[9sh8]], [[Resolution|resolution]] 2.62&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9sh8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9SH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9SH8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.62&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NSS:5-O-[N-(L-ASPARAGINYL)SULFAMOYL]ADENOSINE'>NSS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9sh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9sh8 OCA], [https://pdbe.org/9sh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9sh8 RCSB], [https://www.ebi.ac.uk/pdbsum/9sh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9sh8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SYN_THET8 SYN_THET8]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of Thermus thermophilus asparaginyl-tRNA synthetase has been solved by multiple isomorphous replacement and refined at 2.6 A resolution. This is the last of the three class IIb aminoacyl-tRNA synthetase structures to be determined. As expected from primary sequence comparisons, there are remarkable similarities between the tertiary structures of asparaginyl-tRNA synthetase and aspartyl-tRNA synthetase, and most of the active site residues are identical except for three key differences. The structure at 2.65 A of asparaginyl-tRNA synthetase complexed with a non-hydrolysable analogue of asparaginyl-adenylate permits a detailed explanation of how these three differences allow each enzyme to discriminate between their respective and very similar amino acid substrates, asparagine and aspartic acid. In addition, a structure of the complex of asparaginyl-tRNA synthetase with ATP shows exactly the same configuration of three divalent cations as previously observed in the seryl-tRNA synthetase-ATP complex, showing that this a general feature of class II synthetases. The structural similarity of asparaginyl- and aspartyl-tRNA synthetases as well as that of both enzymes to the ammonia-dependent asparagine synthetase suggests that these three enzymes have evolved relatively recently from a common ancestor.


Authors: Cusack, S., Seignovert, L., Leberman, R., Berthet-Colominas, C.
The crystal structure of asparaginyl-tRNA synthetase from Thermus thermophilus and its complexes with ATP and asparaginyl-adenylate: the mechanism of discrimination between asparagine and aspartic acid.,Berthet-Colominas C, Seignovert L, Hartlein M, Grotli M, Cusack S, Leberman R EMBO J. 1998 May 15;17(10):2947-60. PMID:9582288<ref>PMID:9582288</ref>


Description: Thermus thermophilus asparaginyl-tRNA synthetase with bound sulphamoyl-analogue of asparaginyl-adenylate (AsnAMS)
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Berthet-Colominas, C]]
<div class="pdbe-citations 9sh8" style="background-color:#fffaf0;"></div>
[[Category: Cusack, S]]
== References ==
[[Category: Leberman, R]]
<references/>
[[Category: Seignovert, L]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB8]]
[[Category: Berthet-Colominas C]]
[[Category: Cusack S]]
[[Category: Leberman R]]
[[Category: Seignovert L]]
Retrieved from "https://proteopedia.org/9sh8"