9u9f: Difference between revisions
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==Cryo-EM structure of Oryza sativa phosphate transporter SPDT== | |||
<StructureSection load='9u9f' size='340' side='right'caption='[[9u9f]], [[Resolution|resolution]] 2.77Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9u9f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Indica_Group Oryza sativa Indica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9U9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9U9F FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.77Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9u9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9u9f OCA], [https://pdbe.org/9u9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9u9f RCSB], [https://www.ebi.ac.uk/pdbsum/9u9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9u9f ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A1Y0BRS7_ORYSI A0A1Y0BRS7_ORYSI] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Phosphorus (P) limitation severely affects crop yields. To address the molecular basis of inorganic phosphate (Pi) specific transport within the sulfate transporter (SULTR) family, we determined the cryo-electron microscopy structures of Oryza sativa SULTR-like phosphorus distribution transporter (OsSPDT), a key Pi transporter for grain allocation, in apo- and Pi-binding states. OsSPDT forms a domain-swapped homodimer with each protomer containing an N-terminal domain (NTD), a transmembrane domain (TMD) divided into core and gate subdomains, and a C-terminal sulfate transporter and antisigma factor (STAS) domain. The structure adopts a cytoplasm-facing conformation with Pi coordinated at the core-gate interface. Key residues, including SPDT-unique Ser(170), mediate Pi specificity within the binding pocket, distinguishing it evolutionarily from sulfate transporters within the SULTR family. Domain-swapping and mutational studies demonstrate functional interdependence of the NTD, TMD, and STAS domains. This work elucidates Pi selectivity in plant SULTR transporters and provides a molecular basis for developing low-phytate rice via OsSPDT gene editing. | |||
Molecular mechanism underlying phosphate distribution by SULTR family transporter SPDT in Oryza sativa.,Fang S, Zhao Y, Zhang X, Yu F, Zhang P Sci Adv. 2025 Oct 10;11(41):eady3442. doi: 10.1126/sciadv.ady3442. Epub 2025 Oct , 10. PMID:41071878<ref>PMID:41071878</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9u9f" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Oryza sativa Indica Group]] | |||
[[Category: Fang S]] | |||
[[Category: Zhang P]] | |||
[[Category: Zhang X]] | |||
Latest revision as of 09:25, 22 October 2025
Cryo-EM structure of Oryza sativa phosphate transporter SPDT
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