Halo Tag: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Rupal Yadav (talk | contribs) No edit summary |
Rupal Yadav (talk | contribs) No edit summary |
||
| Line 1: | Line 1: | ||
{{Clear}}<br> | |||
<span style="font-size:160%;line-height:130%;"><b>A "Photoswitchable" HaloTag for Spatiotemporal Control of Fluorescence in Living Cells | |||
<br> | |||
<span style="font-size:120%; line-height:160%;"> Claire Deo, Franziska Walterspiel, Begoña Ugarte-Uribe ''' | |||
<br> | |||
''Scientific Reports'' '''6''':27399, 2025: [https://onlinelibrary.wiley.com/share/ZFYT2R9ZRCUTJRHQZCRW?target=10.1002/anie.202424955]. ([https://doi.org/10.1002/anie.202424955 DOI: 10.1002/anie.202424955]) | |||
</span> | |||
== A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence== | == A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence== | ||
<StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> | <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> | ||
The Photoswitchable Halo Tag (psHaloTag) is a unique chemigenetic system designed to provide reversible, light-controlled fluorescence tunable through both genetic and synthetic modifications, with promising applications for dynamic imaging in biological microscopy <ref>https://doi.org/10.1002/anie.202424955</ref>. psHaloTag addresses a major gap in the availability of reliable, reversible systems suitable for live-cell imaging. Many current systems are limited in tracking long-term, cyclical processes or in refreshing the pool of observable molecules. psHaloTag combines the genetically encoded HaloTag protein with the light-sensing sAsLOV2 domain. When illuminated with 450 nm light, psHaloTag undergoes a reversible change that activates a bound rhodamine dye ligand, resulting in a significant increase in fluorescence. This strong, multiple-cycle reporter overcomes the limitations of irreversible systems. It is an important tool for achieving precise spatiotemporal control in demanding applications such as live-cell Super-Resolution Microscopy (SMLM), where many established photosensitive probes cannot be reactivated. | The Photoswitchable Halo Tag (psHaloTag) is a unique chemigenetic system designed to provide reversible, light-controlled fluorescence tunable through both genetic and synthetic modifications, with promising applications for dynamic imaging in biological microscopy <ref>https://doi.org/10.1002/anie.202424955</ref>. psHaloTag addresses a major gap in the availability of reliable, reversible systems suitable for live-cell imaging. Many current systems are limited in tracking long-term, cyclical processes or in refreshing the pool of observable molecules. psHaloTag combines the genetically encoded HaloTag protein with the light-sensing sAsLOV2 domain. When illuminated with 450 nm light, psHaloTag undergoes a reversible change that activates a bound rhodamine dye ligand, resulting in a significant increase in fluorescence. This strong, multiple-cycle reporter overcomes the limitations of irreversible systems. It is an important tool for achieving precise spatiotemporal control in demanding applications such as live-cell Super-Resolution Microscopy (SMLM), where many established photosensitive probes cannot be reactivated. | ||
