Halo Tag: Difference between revisions
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the dye back to its non-fluorescent state. | the dye back to its non-fluorescent state. | ||
[[Image:Principle of HaloTag.jpg | thumb | upright | | [[Image:Principle of HaloTag.jpg | thumb | upright | right | alt= | Fig.1. General principle of photoswitchable HaloTag (psHaloTag) and open-closed equilibrium of JF635-HaloTag ligand (HTL). L1 and L2 denote linkers. ]] | ||
The psHaloTag variants show a strong, reversible, deep-red fluorescence turn-on when activated by light. This provides a well-controlled tool for improved imaging. | The psHaloTag variants show a strong, reversible, deep-red fluorescence turn-on when activated by light. This provides a well-controlled tool for improved imaging. | ||
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Also, it exploits high-quality synthetic dyes for bright colours and stability in light whose performance does not rely on oxygen and is less affected by pH changes compared to traditional fluorescent proteins (FPs). | Also, it exploits high-quality synthetic dyes for bright colours and stability in light whose performance does not rely on oxygen and is less affected by pH changes compared to traditional fluorescent proteins (FPs). | ||
<Structure load='9HKF' size=' | <Structure load='9HKF' size='200' frame='true' align='right' caption='Crystal structure of psHaloTag labeled with JF635-HTL' | ||
== References == | == References == | ||
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<references/> | <references/> | ||
