Halo Tag: Difference between revisions
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== Introduction == | == Introduction == | ||
<StructureSection load='9HKF' size='340' side='right' caption='Caption for this structure' scene=''> | |||
The [Photoswitchable Halo Tag] (psHaloTag) is a unique chemigenetic system designed to provide reversible, light-controlled fluorescence tunable through both genetic and synthetic modifications, with promising applications for dynamic imaging in biological microscopy <ref>https://doi.org/10.1002/anie.202424955</ref>. psHaloTag addresses a major gap in the availability of reliable, reversible systems suitable for live-cell imaging. Many current systems are limited in tracking long-term, cyclical processes or in refreshing the pool of observable molecules. psHaloTag combines the genetically encoded HaloTag protein with the light-sensing sAsLOV2 domain. When illuminated with 450 nm light, psHaloTag undergoes a reversible change that activates a bound rhodamine dye ligand, resulting in a significant increase in fluorescence. This strong, multiple-cycle reporter overcomes the limitations of irreversible systems. It is an important tool for achieving precise spatiotemporal control in demanding applications such as live-cell Super-Resolution Microscopy (SMLM), where many established photosensitive probes cannot be reactivated. | |||
==== Features ==== | ==== Features ==== | ||
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Also, it exploits high-quality synthetic dyes for bright colours and stability in light whose performance does not rely on oxygen and is less affected by pH changes compared to traditional fluorescent proteins (FPs). | Also, it exploits high-quality synthetic dyes for bright colours and stability in light whose performance does not rely on oxygen and is less affected by pH changes compared to traditional fluorescent proteins (FPs). | ||
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<references/> | <references/> | ||
