HOAT1: Difference between revisions

Line 80:

:::*Path B: Located between TM5 and TM8.

::*This suggests that aromatic

::*This suggests that aromatic residues located at the top border are important for extracellular anion binding, while residues at the bottom play a role in exporting extracellular anions to the cytoplasmic side.

residues located at the top border are important for extracellular

anion binding, while residues at the bottom play a role in

exporting extracellular anions to the cytoplasmic side.

*'''Conformational State:'''

Line 102:

Line 99:

'''2. Key Interacting Residues'''

:*Olmesartan occupies Site 3 of

:*Olmesartan occupies Site 3 of the binding pocket and is located within 5A˚ distance of residues of TM1, TM4, TM5, TM7, TM10, and TM11, namely N35, M207, G227, Y230, W346, Y353, Y354, F438, F442, S462, and R466.

the binding pocket and is located within 5A˚ distance of residues

of TM1, TM4, TM5, TM7, TM10, and TM11, namely N35, M207,

G227, Y230, W346, Y353, Y354, F438, F442, S462, and R466.

:*The

:*The biphenyl group and tetrazole ring of olmesartan rely on interactions with hydrophobic residues close to the bottom gate in the binding pocket.

biphenyl group and tetrazole ring of olmesartan rely on interactions

with hydrophobic residues close to the bottom gate in

the binding pocket.

:*Upon olmesartan binding, the side chain of Y230 undergoes a vertical rotation to accommodate and interact with the substrate.

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@@ Line 80: / Line 80: @@
 :::*Path B: Located between TM5 and TM8.
-::*This suggests that aromatic
+::*This suggests that aromatic residues located at the top border are important for extracellular anion binding, while residues at the bottom play a role in exporting extracellular anions to the cytoplasmic side.
-residues located at the top border are important for extracellular
-anion binding, while residues at the bottom play a role in
-exporting extracellular anions to the cytoplasmic side.
 *'''Conformational State:'''
@@ Line 102: / Line 99: @@
 '''2. Key Interacting Residues'''
-:*Olmesartan occupies Site 3 of
+:*Olmesartan occupies Site 3 of the binding pocket and is located within 5A˚ distance of residues of TM1, TM4, TM5, TM7, TM10, and TM11, namely N35, M207, G227, Y230, W346, Y353, Y354, F438, F442, S462, and R466.
-the binding pocket and is located within 5A˚ distance of residues
-of TM1, TM4, TM5, TM7, TM10, and TM11, namely N35, M207,
-G227, Y230, W346, Y353, Y354, F438, F442, S462, and R466.
-:*The
+:*The biphenyl group and tetrazole ring of olmesartan rely on interactions with hydrophobic residues close to the bottom gate in the binding pocket.
-biphenyl group and tetrazole ring of olmesartan rely on interactions
-with hydrophobic residues close to the bottom gate in
-the binding pocket.
 :*Upon olmesartan binding, the side chain of Y230 undergoes a vertical rotation to accommodate and interact with the substrate.