Halo Tag: Difference between revisions
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*Protein Scaffold: An engineered HaloTag protein with the light-sensing sAsLOV2 domain. | *Protein Scaffold: An engineered HaloTag protein with the light-sensing sAsLOV2 domain. | ||
*Ligand: Binds to a <scene name='10/1096855/ | *Ligand: Binds to a <scene name='10/1096855/Puj/2'>fluorogenic rhodamine dye (JF635-HTL)</scene> | ||
:Light ON (450 nm): Light activates the sAsLOV2 domain using <scene name='10/1096855/Fmn/1'>Flavin Mononucleotide (FMN)</scene> | :Light ON (450 nm): Light activates the sAsLOV2 domain using <scene name='10/1096855/Fmn/1'>Flavin Mononucleotide (FMN)</scene> | ||
