9i2m

Crystal structure of the lipocalin cat allergen Fel d 4

spinqualitylabelsall models 9i2m, resolution 1.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9i2m is a 1 chain structure with sequence from Felis catus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.55Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ALL4_FELCA May be a pheromone carrier. Acts as a kairomone, detected by the prey vomeronasal organ and inducing fear reactions in mice. Publication Abstract from PubMed BACKGROUND: Allergic sensitization to cats and other furry animals is a major cause of asthma and allergic rhinitis in more than 200 million people worldwide. According to the frequency of IgE recognition, allergen-specific IgE levels, and allergenic activity, Fel d 4 is a major allergen in the cat (Felis domesticus). The lipocalin allergen Fel d 4 is highly homologous to dog (Can f 6) and major horse (Equ c 1) allergens. Accordingly, IgE cross-reactivity to these allergens contributes to polysensitization and allergic responses upon exposure to different animals. METHODS: Fel d 4 was recombinantly produced in two systems, E. coli and Expi293F mammalian cells. Recombinant forms were characterized by circular dichroism and mass spectrometry. The Fel d 4 3D structure was determined using X-ray crystallography. Immunoreactivity, epitope analyses, and cross-reactive properties were assessed by ELISA and basophil release assays using allergic patients' sera. RESULTS: We reveal the rFel d 4 crystal structures and demonstrate that mammalian cells produce an N-glycosylated recombinant Fel d 4 allergen. The C-terminal regions of Fel d 4, Can f 6, and Equ c 1 constitute conformational IgE-epitope-containing areas responsible for cross-reactivity. CONCLUSION: Uncovering the IgE-binding sites of Fel d 4 and cross-reactive allergens contributes to future rational design of active and passive allergen-specific treatment forms. Major Cat Allergen Fel d 4: Structure and Identification of a Cross-Reactive IgE-Epitope-Containing Area.,Todorovic N, Trifonova D, Liu Z, Curin M, Schooltink L, Sagmeister T, Grininger C, Kiss R, Gottstein N, Gesslbauer B, Winkler A, Pavkov-Keller T, Karaulov A, Valenta R, Keller W Allergy. 2025 Nov 24. doi: 10.1111/all.70146. PMID:41286560 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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