1x79: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1x79.gif|left|200px]] | [[Image:1x79.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1x79", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_1x79| PDB=1x79 | SCENE= }} | |||
| | |||
| | |||
}} | |||
'''Crystal structure of human GGA1 GAT domain complexed with the GAT-binding domain of Rabaptin5''' | '''Crystal structure of human GGA1 GAT domain complexed with the GAT-binding domain of Rabaptin5''' | ||
| Line 27: | Line 24: | ||
[[Category: Zhang, X C.]] | [[Category: Zhang, X C.]] | ||
[[Category: Zhu, G.]] | [[Category: Zhu, G.]] | ||
[[Category: | [[Category: Gat domain]] | ||
[[Category: | [[Category: Gga protein]] | ||
[[Category: | [[Category: Intracellular trafficking]] | ||
[[Category: | [[Category: Rabaptin5]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:39:28 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 11:39, 3 May 2008
Crystal structure of human GGA1 GAT domain complexed with the GAT-binding domain of Rabaptin5
Overview
GGA proteins coordinate the intracellular trafficking of clathrin-coated vesicles through their interaction with several other proteins. The GAT domain of GGA proteins interacts with ARF, ubiquitin, and Rabaptin5. The GGA-Rabaptin5 interaction is believed to function in the fusion of trans-Golgi-derived vesicles to endosomes. We determined the crystal structure of a human GGA1 GAT domain fragment in complex with the Rabaptin5 GAT-binding domain. In this structure, the Rabaptin5 domain is a 90-residue-long helix. At the N-terminal end, it forms a parallel coiled-coil homodimer, which binds one GAT domain of GGA1. In the C-terminal region, it further assembles into a four-helix bundle tetramer. The Rabaptin5-binding motif of the GGA1 GAT domain consists of a three-helix bundle. Thus, the binding between Rabaptin5 and GGA1 GAT domain is based on a helix bundle-helix bundle interaction. The current structural observation is consistent with previously reported mutagenesis data, and its biological relevance is further confirmed by new mutagenesis studies and affinity analysis. The four-helix bundle structure of Rabaptin5 suggests a functional role in tethering organelles.
About this Structure
1X79 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human GGA1 GAT domain complexed with the GAT-binding domain of Rabaptin5., Zhu G, Zhai P, He X, Wakeham N, Rodgers K, Li G, Tang J, Zhang XC, EMBO J. 2004 Oct 13;23(20):3909-17. Epub 2004 Sep 30. PMID:15457209 Page seeded by OCA on Sat May 3 14:39:28 2008
