9p3u: Difference between revisions
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==Zebrafish TRPM5 E337A mutant with 5mM calcium and 0.5mM CBTA== | |||
<StructureSection load='9p3u' size='340' side='right'caption='[[9p3u]], [[Resolution|resolution]] 2.75Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9p3u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9P3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9P3U FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.75Å</td></tr> | |||
[[Category: | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1CG0:(2~{R},3~{S},4~{S},5~{R},6~{R})-2-(hydroxymethyl)-6-[(2~{R},3~{S},4~{R},5~{R},6~{R})-2-(hydroxymethyl)-6-[(2~{R})-2-(hydroxymethyl)-4-[(1~{R},2~{S},4~{S},5~{R},6~{R},7~{S},8~{R},9~{S},12~{S},13~{S},16~{S},18~{S})-5,7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icosane-6,2-oxane]-16-yl]oxy-butoxy]-4,5-bis(oxidanyl)oxan-3-yl]oxy-oxane-3,4,5-triol'>A1CG0</scene>, <scene name='pdbligand=A1CGW:5-chloro-N-[(5-chloro-1,3-thiazol-2-yl)methyl]-1,2-benzothiazol-6-amine'>A1CGW</scene>, <scene name='pdbligand=A1CGZ:(1~{R},2~{S},4~{S},5~{R},6~{R},7~{S},8~{R},9~{S},12~{S},13~{S},16~{S},18~{S})-5,7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icosane-6,2-oxane]-16-ol'>A1CGZ</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9p3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9p3u OCA], [https://pdbe.org/9p3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9p3u RCSB], [https://www.ebi.ac.uk/pdbsum/9p3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9p3u ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/TRPM5_DANRE TRPM5_DANRE] Monovalent cation-selective ion channel activated by intracellular Ca(2+) in a voltage- and temperature-dependent manner. Mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increase in intracellular Ca(2+), but is impermeable to it. The activation mechanism of TRPM5 involves a multistep process. TRPM5 activation involves ligand binding (i.e., tastant molecule, glucose stimulation) to Gq/G-protein coupled receptors (GPCR) and leads to the breakdown of phosphatidylinositol bisphosphate (PIP2) into diacylglycerol (DAG) and inositol trisphosphate (IP3), IP3 binds to its receptors in the endoplasmic reticulum and cause Ca(2+) release. Simultaneously with the intracellular Ca(2+) release, DAG activates the protein kinase C (PKC), which phosphorylates the TRPM5 channel. This phosphorylation combined with the bound Ca(2+), leads to a robust inward current allowing the entry of sodium ions (Na+) into the cell. This ion influx depolarizes the cell membrane, generating action potentials that propagate TRPM5 signals.[UniProtKB:Q9JJH7][https://www.uniprot.org/uniprot/A0A5P9VSM8_HRSV A0A5P9VSM8_HRSV] | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Danio rerio]] | |||
[[Category: Large Structures]] | |||
[[Category: Du J]] | |||
[[Category: Lu W]] | |||
[[Category: Ruan Z]] | |||
Revision as of 11:40, 7 January 2026
Zebrafish TRPM5 E337A mutant with 5mM calcium and 0.5mM CBTA
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