9zm0: Difference between revisions
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==Crystal structure of monomeric Atg23== | |||
<StructureSection load='9zm0' size='340' side='right'caption='[[9zm0]], [[Resolution|resolution]] 2.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9zm0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9ZM0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9ZM0 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9zm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9zm0 OCA], [https://pdbe.org/9zm0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9zm0 RCSB], [https://www.ebi.ac.uk/pdbsum/9zm0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9zm0 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ATG23_YEAST ATG23_YEAST] Required for cytoplasm to vacuole transport (Cvt) vesicle formation and efficient autophagy. Plays a role in ATG protein retrieval from the pre-autophagosomal structure (PAS) and is especially required for autophagy-dependent cycling of ATG9. Also plays a role in regulation of filamentous growth.<ref>PMID:14504273</ref> <ref>PMID:14723849</ref> <ref>PMID:14734026</ref> <ref>PMID:17426440</ref> <ref>PMID:17700056</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Macroautophagy is a cellular process where cytosolic material is captured in double membrane vesicles, termed autophagosomes, which fuse with the vacuole or lysosomes leading to the degradation of the captured contents. In yeast, the biogenesis of autophagosomes is initiated by the fusion of a few small vesicles which contain the integral membrane protein Atg9. Atg9 vesicle trafficking is in part regulated by the peripheral membrane protein Atg23. However, the structure of Atg23 and the mechanism by which Atg23 interacts with Atg9 are currently unknown. Therefore, we determined the crystal structure for a monomeric form of Atg23 and characterized the interaction between Atg23 and Atg9. This work reveals that Atg23 contains a novel fold which is consistent with the AlphaFold 3 prediction except that the helices running towards the dimerization region have a bend giving a more curved global architecture than the prediction. In addition, we demonstrate that conserved sequences in both the N and C-terminal regions of Atg9 bind to a hydrophobic cavity on Atg23. | |||
Atg23 Interacts With Both the N- and C-termini of Atg9 Via a Hydrophobic Binding Pocket.,Bekkhozhin Z, Leary KA, Ragusa MJ bioRxiv [Preprint]. 2025 Dec 19:2025.12.17.694986. doi: , 10.64898/2025.12.17.694986. PMID:41446224<ref>PMID:41446224</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9zm0" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharomyces cerevisiae]] | |||
[[Category: Bekkhozhin Z]] | |||
[[Category: Ragusa MJ]] | |||
