9q73: Difference between revisions

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'''Unreleased structure'''


The entry 9q73 is ON HOLD
==Crystal structure of T. brucei EIF4E5 in complex with EIF4G1 peptide==
<StructureSection load='9q73' size='340' side='right'caption='[[9q73]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9q73]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei_TREU927 Trypanosoma brucei brucei TREU927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9Q73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9Q73 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1BO:1-BUTANOL'>1BO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PDO:1,3-PROPANDIOL'>PDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9q73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9q73 OCA], [https://pdbe.org/9q73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9q73 RCSB], [https://www.ebi.ac.uk/pdbsum/9q73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9q73 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q38B99_TRYB2 Q38B99_TRYB2]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Translation initiation in eukaryotes is a highly regulated process involving the assembly of several transient protein complexes. A key step in this process is recognition of the mRNA 5' cap structure by the initiation factor eIF4E, a core component of the eIF4F complex. In trypanosomatids, this mechanism diverges from canonical eukaryotic systems, featuring five distinct eIF4F-like complexes formed through specific pairings of eIF4E and eIF4G homologs. Additionally, trypanosomatid mRNAs exhibit a unique hypermethylated cap-4 structure at the 5' end. To elucidate the molecular basis of selective eIF4E-eIF4G interactions and the modulation of cap binding upon eIF4G engagement, we determined high-resolution crystal structures of EIF4E5-EIF4G1 complexes from Trypanosoma brucei and T. cruzi, and the EIF4E6-EIF4G5 complex from T. cruzi. These structural studies, supported by biophysical analyses in the presence and absence of a cap-4 analog, reveal key determinants of cap recognition associated with cap-4 structural flexibility and plasticity in the cap-binding pockets. We observe conformational rearrangements upon eIF4G binding and propose a relationship between these structural changes and increased cap-4 affinity. In addition, comparative structural analysis of the EIF4E5-EIF4G1 and EIF4E6-EIF4G5 complexes offers atomic-level insights into the molecular determinants of specificity that govern selective eIF4E-eIF4G pairings in trypanosomatids.


Authors: Penteado, R.F., Guimaraes, B.G.
Selective eIF4E-eIF4G Pairing and Cap-4 Recognition Mechanisms in Trypanosomatids: Insights From EIF4E5-EIF4G1 and EIF4E6-EIF4G5 Complexes.,Penteado RF, Vichier-Guerre S, da Silva Pereira BM, Dugue L, Guerra Slompo EP, Assuncao de Matos TR, Pochet S, Zanchin NIT, Guimaraes BG J Mol Biol. 2026 Jan 15;438(2):169550. doi: 10.1016/j.jmb.2025.169550. Epub 2025 , Nov 18. PMID:41265746<ref>PMID:41265746</ref>


Description: Crystal structure of T. brucei EIF4E5 in complex with EIF4G1 peptide
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Guimaraes, B.G]]
<div class="pdbe-citations 9q73" style="background-color:#fffaf0;"></div>
[[Category: Penteado, R.F]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Trypanosoma brucei brucei TREU927]]
[[Category: Guimaraes BG]]
[[Category: Penteado RF]]
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