9q78: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 9q78 is ~~ON HOLD~~

==Crystal structure of T. cruzi EIF4E6 in complex with EIF4G5 peptide==

== Structural highlights ==

<table><tr><td colspan='2'>[[9q78]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi] and [https://en.wikipedia.org/wiki/Trypanosoma_cruzi_Dm28c Trypanosoma cruzi Dm28c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9Q78 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9Q78 FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9q78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9q78 OCA], [https://pdbe.org/9q78 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9q78 RCSB], [https://www.ebi.ac.uk/pdbsum/9q78 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9q78 ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/A0A2V2VTW2_TRYCR A0A2V2VTW2_TRYCR]

== Publication Abstract from PubMed ==

Translation initiation in eukaryotes is a highly regulated process involving the assembly of several transient protein complexes. A key step in this process is recognition of the mRNA 5' cap structure by the initiation factor eIF4E, a core component of the eIF4F complex. In trypanosomatids, this mechanism diverges from canonical eukaryotic systems, featuring five distinct eIF4F-like complexes formed through specific pairings of eIF4E and eIF4G homologs. Additionally, trypanosomatid mRNAs exhibit a unique hypermethylated cap-4 structure at the 5' end. To elucidate the molecular basis of selective eIF4E-eIF4G interactions and the modulation of cap binding upon eIF4G engagement, we determined high-resolution crystal structures of EIF4E5-EIF4G1 complexes from Trypanosoma brucei and T. cruzi, and the EIF4E6-EIF4G5 complex from T. cruzi. These structural studies, supported by biophysical analyses in the presence and absence of a cap-4 analog, reveal key determinants of cap recognition associated with cap-4 structural flexibility and plasticity in the cap-binding pockets. We observe conformational rearrangements upon eIF4G binding and propose a relationship between these structural changes and increased cap-4 affinity. In addition, comparative structural analysis of the EIF4E5-EIF4G1 and EIF4E6-EIF4G5 complexes offers atomic-level insights into the molecular determinants of specificity that govern selective eIF4E-eIF4G pairings in trypanosomatids.

~~Authors~~: Penteado, R.F., ~~Guimaraes, B.G~~.

Selective eIF4E-eIF4G Pairing and Cap-4 Recognition Mechanisms in Trypanosomatids: Insights From EIF4E5-EIF4G1 and EIF4E6-EIF4G5 Complexes.,Penteado RF, Vichier-Guerre S, da Silva Pereira BM, Dugue L, Guerra Slompo EP, Assuncao de Matos TR, Pochet S, Zanchin NIT, Guimaraes BG J Mol Biol. 2026 Jan 15;438(2):169550. doi: 10.1016/j.jmb.2025.169550. Epub 2025 , Nov 18. PMID:41265746<ref>PMID:41265746</ref>

~~Description: Crystal structure~~ of T. cruzi ~~EIF4E6 in complex with EIF4G5 peptide~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased Structures~~]]

</div>

[[Category: Guimaraes~~, B.G~~]]

[[Category: Penteado~~, R.F~~]]

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Trypanosoma cruzi]]

[[Category: Trypanosoma cruzi Dm28c]]

[[Category: Guimaraes BG]]

[[Category: Penteado RF]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9q78 is ON HOLD
+==Crystal structure of T. cruzi EIF4E6 in complex with EIF4G5 peptide==
+<StructureSection load='9q78' size='340' side='right'caption='[[9q78]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9q78]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi] and [https://en.wikipedia.org/wiki/Trypanosoma_cruzi_Dm28c Trypanosoma cruzi Dm28c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9Q78 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9Q78 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9q78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9q78 OCA], [https://pdbe.org/9q78 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9q78 RCSB], [https://www.ebi.ac.uk/pdbsum/9q78 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9q78 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A2V2VTW2_TRYCR A0A2V2VTW2_TRYCR]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Translation initiation in eukaryotes is a highly regulated process involving the assembly of several transient protein complexes. A key step in this process is recognition of the mRNA 5' cap structure by the initiation factor eIF4E, a core component of the eIF4F complex. In trypanosomatids, this mechanism diverges from canonical eukaryotic systems, featuring five distinct eIF4F-like complexes formed through specific pairings of eIF4E and eIF4G homologs. Additionally, trypanosomatid mRNAs exhibit a unique hypermethylated cap-4 structure at the 5' end. To elucidate the molecular basis of selective eIF4E-eIF4G interactions and the modulation of cap binding upon eIF4G engagement, we determined high-resolution crystal structures of EIF4E5-EIF4G1 complexes from Trypanosoma brucei and T. cruzi, and the EIF4E6-EIF4G5 complex from T. cruzi. These structural studies, supported by biophysical analyses in the presence and absence of a cap-4 analog, reveal key determinants of cap recognition associated with cap-4 structural flexibility and plasticity in the cap-binding pockets. We observe conformational rearrangements upon eIF4G binding and propose a relationship between these structural changes and increased cap-4 affinity. In addition, comparative structural analysis of the EIF4E5-EIF4G1 and EIF4E6-EIF4G5 complexes offers atomic-level insights into the molecular determinants of specificity that govern selective eIF4E-eIF4G pairings in trypanosomatids.
-Authors: Penteado, R.F., Guimaraes, B.G.
+Selective eIF4E-eIF4G Pairing and Cap-4 Recognition Mechanisms in Trypanosomatids: Insights From EIF4E5-EIF4G1 and EIF4E6-EIF4G5 Complexes.,Penteado RF, Vichier-Guerre S, da Silva Pereira BM, Dugue L, Guerra Slompo EP, Assuncao de Matos TR, Pochet S, Zanchin NIT, Guimaraes BG J Mol Biol. 2026 Jan 15;438(2):169550. doi: 10.1016/j.jmb.2025.169550. Epub 2025 , Nov 18. PMID:41265746<ref>PMID:41265746</ref>
-Description: Crystal structure of T. cruzi EIF4E6 in complex with EIF4G5 peptide
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Guimaraes, B.G]]
+<div class="pdbe-citations 9q78" style="background-color:#fffaf0;"></div>
-[[Category: Penteado, R.F]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Trypanosoma cruzi]]
+[[Category: Trypanosoma cruzi Dm28c]]
+[[Category: Guimaraes BG]]
+[[Category: Penteado RF]]