9n9a: Difference between revisions
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==Crystal structure of Class-3 OPX WzaB from Myxococcus xanthus== | |||
<StructureSection load='9n9a' size='340' side='right'caption='[[9n9a]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9n9a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus_DZ2 Myxococcus xanthus DZ2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9N9A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9N9A FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9n9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9n9a OCA], [https://pdbe.org/9n9a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9n9a RCSB], [https://www.ebi.ac.uk/pdbsum/9n9a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9n9a ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial secretion of extracellular polysaccharides is essential for surface colonization, biofilm formation, and pathogenesis. In diderm bacteria, such polymers traverse the periplasm and outer membrane (OM) through outer-membrane polysaccharide export (OPX) proteins that form secretion pores. Among them, Class-3 OPX proteins are the most widespread but lack an OM-spanning pore domain, leaving their mechanisms poorly understood. Here, we characterize WzaB from Myxococcus xanthus as a model for Class-3 OPX-mediated secretion. Structural and molecular dynamics analyses reveal that WzaB exists as a rigid monomer in solution, in contrast to the constitutive octamerization observed in Class-1 OPX proteins. Biochemical, biophysical, and in vivo analyses show that WzaB oligomerizes in a lipidation-dependent manner and directly interacts with the OM porin WzpB and the inner-membrane co-polymerase WzcB, with binding determinants mapped for both partners. Together, these proteins assemble into a trans-envelope polysaccharide secretion complex, redefining OPX function and revealing a distinct translocon architecture for Class-3 OPX systems. | |||
Mechanism of trans-envelope bacterial polysaccharide secretion in Class-3 outer-membrane polysaccharide export (OPX) protein systems.,Mellouk A, Ngo K, Kezzo AA, Martini C, Franca TCC, Laplante SR, Islam ST, Calmettes C Nat Commun. 2026 Jan 16;17(1):732. doi: 10.1038/s41467-025-67321-3. PMID:41545361<ref>PMID:41545361</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9n9a" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Myxococcus xanthus DZ2]] | |||
[[Category: Calmettes C]] | |||
[[Category: Mellouk A]] | |||
Latest revision as of 14:36, 10 February 2026
Crystal structure of Class-3 OPX WzaB from Myxococcus xanthus
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