9snd

Mus musculus acetylcholinesterase in complex with 2-(1H-indol-3-yl)-N-(2-methoxybenzyl)ethan-1-amine

spinqualitylabelsall models 9snd, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9snd is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.4Å Ligands: 9YU, A1JOW, PG0, TOE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ACES_MOUSE Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Publication Abstract from PubMed Vector control with insecticides is an important preventive measure against mosquito-borne infectious diseases, such as malaria and dengue. The intensive usage of few insecticides has resulted in emerging resistance in mosquitoes, and unwanted off-target toxic effects. Therefore, there is great interest in alternative active ingredients. Here, we explore indole-based compounds as selective inhibitors against acetylcholinesterase 1 (AChE1) from the disease-transmitting mosquitoes Anopheles gambiae (An. gambiae, AgAChE1) and Aedes aegypti (Ae. aegypti, AeAChE1) as potential candidates for future insecticides used in vector control. Three sets of compounds were designed to explore their structure-activity relationship, and investigate their potentials regarding potency and selectivity. 26 indole-based compounds were synthesized and biochemically evaluated for inhibition against AgAChE1, AeAChE1, and human AChE (hAChE). The compounds were shown to be potent inhibitors against AChE1, and selective for AChE1 over hAChE. N-Methylation of the indole moiety clearly increased the inhibition potency, and a bulkier benzyl moiety improved the selectivity. X-ray crystallography shows that the inhibitors bind at the bottom of the active site gorge of mouse AChE (mAChE), while molecular dynamics simulations revealed different binding poses in mAChE and AgAChE1. Four potent and selective inhibitors were subjected to in vivo mosquito testing. Topical application showed strong insecticidal effects on An. gambiae and Ae. aegypti, highlighting this compound class as an interesting alternative for future insecticide research. Potent and selective indole-based inhibitors targeting disease-transmitting mosquitoes.,Rajeshwari R, Duvauchelle V, Lindgren C, Stangner K, Knutsson S, Forsgren N, Ekstrom F, Kamau L, Linusson A RSC Med Chem. 2025 Nov 19. doi: 10.1039/d5md00797f. PMID:41551022 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA