9y6v: Difference between revisions
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==attPsym bound large serine integrase and RDF complex in the dimeric state== | |||
<StructureSection load='9y6v' size='340' side='right'caption='[[9y6v]], [[Resolution|resolution]] 7.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9y6v]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_SPbeta Bacillus virus SPbeta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9Y6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9Y6V FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.1Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9y6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9y6v OCA], [https://pdbe.org/9y6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9y6v RCSB], [https://www.ebi.ac.uk/pdbsum/9y6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9y6v ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Large serine integrases (LSIs) catalyze unidirectional site-specific DNA recombination reactions, yet those reactions are reversed by the presence of a cognate recombination directionality factor (RDF). Mechanistic understanding of directionality control has been hampered by a lack of structural information. Here, we use cryo-electron microscopy (cryo-EM) to determine the structures of six SPbeta integrase-DNA complexes along the integrative (-RDF) and excisive (+RDF) reaction pathways, at 4.16-7.18A resolution. Our findings reveal how RDF-mediated repositioning of an integrase subdomain (1) dictates which pairs of DNA sites can be assembled into a synaptic complex to initiate recombination and (2) dictates which product complexes will be conformationally locked, preventing the back reaction. These mechanistic insights provide a conceptual framework for engineering efficient and versatile genome editing tools. | |||
Structural basis of directionality control in large serine integrases.,Shin H, Pigli Y, Reyes TP, Fuller JR, Olorunniji FJ, Rice PA bioRxiv [Preprint]. 2025 Jan 13:2025.01.03.631226. doi: , 10.1101/2025.01.03.631226. PMID:39803483<ref>PMID:39803483</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9y6v" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bacillus virus SPbeta]] | |||
[[Category: Large Structures]] | |||
[[Category: Olorunniji FJ]] | |||
[[Category: Rice PA]] | |||
[[Category: Shin H]] | |||
Latest revision as of 19:36, 10 February 2026
attPsym bound large serine integrase and RDF complex in the dimeric state
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