9h2m: Difference between revisions
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==Cas9:crRNA:tracrRNA in complex with PAM-containing non-cognate DNA, PAM-unbound conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system== | |||
<StructureSection load='9h2m' size='340' side='right'caption='[[9h2m]], [[Resolution|resolution]] 3.06Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9h2m]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_thermophilus_DGCC_7710 Streptococcus thermophilus DGCC 7710] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9H2M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9H2M FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.06Å</td></tr> | |||
[[Category: | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9h2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9h2m OCA], [https://pdbe.org/9h2m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9h2m RCSB], [https://www.ebi.ac.uk/pdbsum/9h2m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9h2m ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CAS9_STRTR CAS9_STRTR] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA (Probable). Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer yielding blunt ends; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes a 3'-G-rich protospacer adjacent motif (PAM, TGGTG in this organism) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity. When the CRISPR3/cas system consisting of cas9-cas1-cas2-csn2-CRISPR3 or just cas9-CRISPR3 is expressed in E.coli it prevents plasmids homologous to spacers 1 or 2 from transforming.<ref>PMID:21813460</ref> <ref>PMID:22949671</ref> <ref>PMID:23535272</ref> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptococcus thermophilus DGCC 7710]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Gaizauskaite U]] | |||
[[Category: Sasnauskas G]] | |||
[[Category: Tamulaitiene G]] | |||
Latest revision as of 07:03, 18 February 2026
Cas9:crRNA:tracrRNA in complex with PAM-containing non-cognate DNA, PAM-unbound conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system
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