9skh: Difference between revisions
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==Biocatalytic Regioselective C-Formylation of Resorcinol Derivatives== | |||
<StructureSection load='9skh' size='340' side='right'caption='[[9skh]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9skh]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Chromobacterium_sphagni Chromobacterium sphagni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9SKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9SKH FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9skh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9skh OCA], [https://pdbe.org/9skh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9skh RCSB], [https://www.ebi.ac.uk/pdbsum/9skh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9skh ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Although aromatic formylation reactions are highly valuable from a synthetic perspective, a biocatalytic version has not yet been reported. Here, the cofactor-independent multimeric three-component acyltransferase from Chromobacterium sphagni (CsATase) was identified to enable the nonnatural promiscuous regioselective C-formylation of polyphenolic substrates, especially resorcinol derivatives, and thus extending the reaction scope of acyltransferases. Formylation of 4- and 5-substituted resorcinol derivatives gave access to regioselectively mono-formylated products with up to 99% conversion and up to 74% isolated yield. Formylation of phloroglucinol led to the di-formylated product with 99% conversion, outperforming chemical methods. Structural analysis of CsATase by X-ray crystallography provided insights into its active site. | |||
Biocatalytic Regioselective C-Formylation of Resorcinol Derivatives.,Gal L, Rohan S, Zadlo-Dobrowolska A, Hilweg B, Muller J, Tittmann K, Kroutil W Angew Chem Int Ed Engl. 2026 Jan 29:e19387. doi: 10.1002/anie.202519387. PMID:41612625<ref>PMID:41612625</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9skh" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Chromobacterium sphagni]] | |||
[[Category: Large Structures]] | |||
[[Category: Gal L]] | |||
[[Category: Kroutil W]] | |||
[[Category: Rohan S]] | |||
[[Category: Tittmann K]] | |||
Latest revision as of 07:18, 18 February 2026
Biocatalytic Regioselective C-Formylation of Resorcinol Derivatives
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