9y44: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Structure of naked mole-rat ribosome (rotated, tRNAs, and mRNA)== | |||
<StructureSection load='9y44' size='340' side='right'caption='[[9y44]], [[Resolution|resolution]] 4.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9y44]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Heterocephalus_glaber Heterocephalus glaber]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9Y44 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9Y44 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.9Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MLZ:N-METHYL-LYSINE'>MLZ</scene>, <scene name='pdbligand=NMM:(2S)-2-AMINO-5-[(N-METHYLCARBAMIMIDOYL)AMINO]PENTANOIC+ACID'>NMM</scene>, <scene name='pdbligand=V5N:(2~{S},3~{S})-2-azanyl-3-(1~{H}-imidazol-5-yl)-3-oxidanyl-propanoic+acid'>V5N</scene>, <scene name='pdbligand=YYG:4-(3-[5-O-PHOSPHONORIBOFURANOSYL]-4,6-DIMETHYL-8-OXO-4,8-DIHYDRO-3H-1,3,4,5,7A-PENTAAZA-S-INDACEN-YLAMINO-BUTYRIC+ACID+METHYL+ESTER'>YYG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9y44 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9y44 OCA], [https://pdbe.org/9y44 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9y44 RCSB], [https://www.ebi.ac.uk/pdbsum/9y44 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9y44 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ribosomes are central to protein synthesis in all organisms. In mammals, the ribosome functional core is highly conserved. Remarkably, two rodent species, the naked mole-rat (NMR) and tuco-tuco, display fragmented 28S ribosomal RNA (rRNA), coupled with high translational fidelity and long lifespan. The unusual ribosomal architecture in the NMR and tuco-tuco has been speculated to be linked to high translational fidelity. Here, we show, by single-particle cryo-electron microscopy, that despite the fragmentation of their rRNA, NMR and tuco-tuco ribosomes retain their core functional architecture. Compared to ribosomes of the guinea pig, a phylogenetically related rodent without 28S rRNA fragmentation, ribosomes of NMR and tuco-tuco exhibit poorly resolved density for certain expansion segments. In contrast, the structure of the guinea pig ribosome shows high similarity to the human ribosome. Enhanced translational fidelity in the NMR and tuco-tuco may stem from subtle, allosteric effects in dynamics, linked to rRNA fragmentation. | |||
Structures of naked mole-rat, tuco-tuco, and guinea pig ribosomes-is rRNA fragmentation linked to translational fidelity?,Gutierrez-Vargas C, De S, Maji S, Liu Z, Ke Z, Niess M, Seluanov A, Gorbunova V, Frank J Nucleic Acids Res. 2026 Jan 22;54(3):gkag006. doi: 10.1093/nar/gkag006. PMID:41603730<ref>PMID:41603730</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 9y44" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Gorbunova | __TOC__ | ||
[[Category: Liu | </StructureSection> | ||
[[Category: | [[Category: Heterocephalus glaber]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: De S]] | ||
[[Category: Frank J]] | |||
[[Category: Gorbunova V]] | |||
[[Category: Gutierrez-Vargas C]] | |||
[[Category: Liu Z]] | |||
[[Category: Maji S]] | |||
[[Category: Nieb M]] | |||
[[Category: Seluanov A]] | |||
Latest revision as of 07:27, 18 February 2026
Structure of naked mole-rat ribosome (rotated, tRNAs, and mRNA)
| ||||||||||||
