9mvs: Difference between revisions

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'''Unreleased structure'''


The entry 9mvs is ON HOLD  until Paper Publication
==Activated Leptotrichia buccalis (Lbu) CRISPR-Cas13a bound to AI-designed anti-CRISPR AIcrVIA1==
 
<StructureSection load='9mvs' size='340' side='right'caption='[[9mvs]], [[Resolution|resolution]] 3.43&Aring;' scene=''>
Authors:  
== Structural highlights ==
 
<table><tr><td colspan='2'>[[9mvs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptotrichia_buccalis Leptotrichia buccalis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9MVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9MVS FirstGlance]. <br>
Description:  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.43&#8491;</td></tr>
[[Category: Unreleased Structures]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9mvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9mvs OCA], [https://pdbe.org/9mvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9mvs RCSB], [https://www.ebi.ac.uk/pdbsum/9mvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9mvs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CS13A_LEPBD CS13A_LEPBD] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements (spacer sequences) and target invading nucleic acids. Unlike many single-component effectors, this CRISPR-Cas system targets RNA (PubMed:27669025). CRISPR clusters are transcribed from pre-CRISPR RNA (crRNA) and processed into crRNA by this protein (PubMed:27669025, PubMed:28475872, PubMed:28757251). pre-crRNA processing yields a 5'-OH and probably a 2',3'-cyclic phosphate (PubMed:27669025). Also cleaves pre-crRNA from several other type VI-A CRISPR systems (PubMed:28475872). Cleaves linear target ssRNA in a crRNA-dependent fashion, preferentially before U residues (PubMed:27669025, PubMed:28475872). Cleavage of target ssRNA is about 80-fold faster than pre-crRNA processing and uses a different active site (PubMed:27669025). Binding a viable target RNA target activates this protein for non-specific RNA degradation in vitro (called collateral RNA degradation) (PubMed:27669025, PubMed:28475872, PubMed:28757251). Activation occurs with 10 fM target RNA (PubMed:28475872). crRNA maturation is not essential for activation of RNA degradation, but lack of mature crRNA (due to mutagenesis) decreases activation levels (PubMed:28475872). This system has a 3' protospacer flanking site in the target RNA (PFS), which is C and unavailable to base pair with crRNA (PFS is equivalent to PAM, the protospacer adjacent motif) (PubMed:28757251).<ref>PMID:27669025</ref> <ref>PMID:28475872</ref> <ref>PMID:28757251</ref>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Leptotrichia buccalis]]
[[Category: Synthetic construct]]
[[Category: Knott GJ]]
[[Category: Taveneau C]]
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